# US 2019/0375824 A1 · Vhh antibody

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- Number: US 2019/0375824 A1
- Document ID: US20190375824A1
- Status: Published application
- Kind: A1
- Application number: 16/372,506
- Filed: 2019-04-02
- Published: 2019-12-12
- Earliest claimed: 2018-06-08
- Assignees: Panasonic Intellectual Property Management Co., Ltd.
- Applicants: Panasonic Intellectual Property Management Co., Ltd
- Inventors: EMINA IKEUCHI; JIN MURAOKA
- Web page: https://patentgrep.com/patent/US20190375824A1

## CPC

- C07K 16/065: {Purification, fragmentation}
- C07K 16/18: against material from animals or humans
- C12N 15/85: for animal cells
- C07K 2317/51: Complete heavy chain or Fd fragment, i.e. VH + CH1
- C07K 2317/569: Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
- C07K 2317/92: Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
- C07K 2317/94: Stability, e.g. half-life, pH, temperature or enzyme-resistance
- C12N 2015/8518: {expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Provided is a VHH antibody including an amino acid sequence in which at least one selected from the group consisting of glycine in position 36, arginine in position 50, and glycine in positon 78, on the basis of IMGT numbering, of the amino acid sequence represented by SEQ ID NO: 1 is substituted with another amino acid. The VHH antibody according to the present disclosure is thermostable.

## Claims

### Claim 1

A VHH antibody including an amino acid sequence in which at least one selected from the group consisting of glycine in position 36, arginine in position 50, and glycine in positon 78, on the basis of IMGT numbering, of the amino acid sequence represented by SEQ ID NO: 1 is substituted with another amino acid.

### Claim 2 (depends on 1)

The VHH antibody according to claim 1, wherein
the glycine in position 36 is substituted with one amino acid selected from the group consisting of aspartic acid, glutaminic acid, arginine, lysin, histidine, asparagine and glutamine.

### Claim 3 (depends on 1)

The VHH antibody according to claim 1, wherein
the arginine in positon 50 is substituted with one amino acid selected from the group consisting of alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan, cysteine, serine, threonine, asparagine, glutamine and tyrosine.

### Claim 4 (depends on 1)

The VHH antibody according to claim 1, wherein
the glycine in position 78 is substituted with one amino acid selected from the group consisting of alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan, cysteine, aspartic acid, glutaminic acid, arginine, lysin, histidine, serine, threonine, asparagine, glutamine and tyrosine.

### Claim 5 (depends on 1)

The VHH antibody according to claim 1, wherein
serine in position 116 is substituted with another amino acid.

### Claim 6 (depends on 5)

The VHH antibody according to claim 5, wherein
the serine in position 116 is substituted with one amino acid selected from the group consisting of aspartic acid, glutaminic acid, arginine, lysin, histidine, asparagine and glutamine.

### Claim 7

A VHH antibody including an amino acid sequence represented by any one selected from the group consisting of SEQ ID NO: 3-SEQ ID NO: 12.