# US 2019/0375804 A1 · Modular resilin-mimetic elastomeric platform > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0375804 A1 - Document ID: US20190375804A1 - Status: Published application - Kind: A1 - Application number: 16/349,065 - Filed: 2017-11-17 - Published: 2019-12-12 - Earliest claimed: 2016-11-18 - Assignees: Pandorum Technologies Private Limited - Applicants: Pandorum Technologies Private Limited - Inventors: Tuhin BHOWMICK; Arun Chandru RAJA - Web page: https://patentgrep.com/patent/US20190375804A1 ## CPC - C07K 14/43563: {from insects} - C12N 9/00: Enzymes; Proenzymes; Compositions thereof (preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; {enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113}); Processes for preparing, activating, inhibiting, separating or purifying enzymes (preparation of malt C12C1/00) - C12N 15/70: Vectors or expression systems specially adapted for E. coli - C12P 21/02: having a known sequence of two or more amino acids, e.g. glutathione - C07K 2319/00: Fusion polypeptide - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} ## Abstract Disclosed herein is a synthetic polypeptide with “n” number of repeats of a sequence as set forth in SEQ ID NO: 1 or SEQ ID NO: 2. The synthetic polypeptide as disclosed herein is represented by an amino acid sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 4, or SEQ ID NO: 5. The synthetic polypeptide of the present disclosure is used to prepare synthetic elastomeric hydrogel. Also, disclosed are the methods of preparing the synthetic polypeptide and the elastomeric hydrogel along with their uses. ## Claims ### Claim 1 A synthetic polypeptide comprising “n” number of repeats of a sequence as set forth in SEQ ID NO: 1 or SEQ ID NO: 2. ### Claim 2 (depends on 1) The synthetic polypeptide as claimed in claim 1 having sequence as set forth in SEQ ID NO: 3, SEQ ID NO: 4, or SEQ ID NO: 5. ### Claim 3 (depends on 2) The synthetic polypeptide as claimed in claim 2, encoded by a polynucleotide sequence as set forth in SEQ ID NO: 6, SEQ ID NO: 7, or SEQ ID NO: 8. ### Claim 4 (depends on 1) A DNA construct comprising a polynucleotide fragment encoding a synthetic polypeptide as claimed in claim 1, operably linked to a promoter. ### Claim 5 (depends on 4) The DNA construct as claimed in claim 4, wherein said polynucleotide fragment encodes a synthetic polypeptide having sequence as set forth in SEQ ID NO: 3, SEQ ID NO: 4, or SEQ ID NO: 5. ### Claim 6 (depends on 5) The DNA construct as claimed in claim 5, wherein said polynucleotide fragment sequence is as set forth in SEQ ID NO: 6, SEQ ID NO: 7, or SEQ ID NO: 8. ### Claim 7 (depends on 4) A DNA vector comprising a DNA construct as claimed in claim 4. ### Claim 8 (depends on 4) A recombinant host cell comprising a DNA construct as claimed in claim 4. ### Claim 9 (depends on 8) The recombinant host cell as claimed in claim 8, wherein said host cell is of bacterial, plant, fungal, insect, or mammalian origin. ### Claim 10 (depends on 9) The recombinant host cell as claimed in claim 9, wherein said host cell is E. coli. ### Claim 11 (depends on 8) A method of obtaining a synthetic peptide, said method comprising: a. obtaining a recombinant host cell as claimed in claim 8; b. culturing said recombinant host cell under conditions conducive for expression of a synthetic peptide; and c. isolating and purifying said synthetic peptide. ### Claim 12 (depends on 1) An elastomeric hydrogel comprising a synthetic peptide as claimed in claim 1. ### Claim 13 (depends on 12) A method of obtaining an elastomeric hydrogel as claimed in claim 12. ### Claim 14 (depends on 1) A synthetic peptide as claimed in claim 1 for use in preparing elastomeric hydrogels. ### Claim 15 (depends on 12) An elastomeric hydrogel as claimed in claim 12 for use in bio-medical applications.