# US 2019/0375802 A1 · Novel nucleoside triphosphate transporter and uses thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0375802 A1 - Document ID: US20190375802A1 - Status: Published application - Kind: A1 - Application number: 16/546,097 - Filed: 2019-08-20 - Published: 2019-12-12 - Earliest claimed: 2016-06-24 - Assignees: The Scripps Research Institute - Applicants: The Scripps Research Institute - Inventors: Floyd E. ROMESBERG; Yorke ZHANG - Web page: https://patentgrep.com/patent/US20190375802A1 ## CPC - C07K 14/405: from algae - C12N 9/22: Ribonucleases {[RNase]; Deoxyribonucleases [DNase]} - C12N 9/224: {Class 1 CAS enzyme complex, e.g. multi-enzyme} - C12N 15/10: Processes for the isolation, preparation or purification of DNA or RNA (chemical preparation of DNA or RNA C07H21/00; preparation of non-structural polynucleotides from microorganisms or with enzymes C12P19/34) - C12N 15/11: DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} - C12N 15/90: Stable introduction of foreign DNA into chromosome - C12N 2310/20: involving clustered regularly interspaced short palindromic repeats [CRISPR] - C12N 2800/80: Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract Disclosed herein are proteins, methods, cells, engineered microorganisms, and kits for generating a modified nucleoside triphosphate transporter from Phaeodactylum tricornutum. Also disclosed herein proteins, methods, cells, engineered microorganisms, and kits for production of a nucleic acid molecule that comprises an unnatural nucleotide utilizing a modified nucleoside triphosphate transporter from Phaeodactylum tricornutum. ## Claims ### Claim 1 A recombinant nucleoside triphosphate transporter from Phaeodactylum tricornutum with an amino acid sequence comprising a deletion. ### Claim 2 (depends on 1) The recombinant nucleoside triphosphate transporter of claim 1, wherein the deletion comprises about 5, 10, 15, 20, 22, 25, 30, 40, 44, 50, 60, 65, 66, 70, or more amino acid residues. ### Claim 3 (depends on 1) The recombinant nucleoside triphosphate transporter of claim 1, wherein the deletion is a N-terminal deletion. ### Claim 4 (depends on 1) The recombinant nucleoside triphosphate transporter of claim 1, wherein the amino acid sequence is at least 80% identical to SEQ ID NO: 4. ### Claim 5 (depends on 1) The recombinant nucleoside triphosphate transporter of claim 1, wherein the amino acid sequence is at least 85% identical to SEQ ID NO: 4. ### Claim 6 (depends on 1) The recombinant nucleoside triphosphate transporter of claim 1, wherein the amino acid sequence is at least 90% identical to SEQ ID NO: 4. ### Claim 7 (depends on 1) The recombinant nucleoside triphosphate transporter of claim 1, wherein the amino acid sequence is at least 95% identical to SEQ ID NO: 4. ### Claim 8 (depends on 1) The recombinant nucleoside triphosphate transporter of claim 1, wherein the amino acid sequence is at least 99% identical to SEQ ID NO: 4. ### Claim 9 (depends on 1) The recombinant nucleoside triphosphate transporter of claim 1, wherein the amino acid sequence is 100% identical to SEQ ID NO: 4. ### Claim 10 (depends on 1) The recombinant nucleoside triphosphate transporter of claim 1, wherein the recombinant nucleoside triphosphate transporter is encoded by an extrachromosomal nucleic acid molecule. ### Claim 11 (depends on 10) The recombinant nucleoside triphosphate transporter of claim 10, wherein the extrachromosomal nucleic acid molecule comprises a nucleic acid sequence provided in SEQ ID NO: 2. ### Claim 12 (depends on 1) A cell comprising the recombinant nucleoside triphosphate transporter of claim 1. ### Claim 13 (depends on 12) The cell of claim 12, wherein the cell is a prokaryotic cell. ### Claim 14 (depends on 12) The cell of claim 12, wherein the cell is an engineered cell. ### Claim 15 (depends on 12) The cell of claim 12, wherein the cell is E. coli. ### Claim 16 (depends on 12) The cell of claim 12, further comprising an unnatural nucleoside triphosphate transported into the cell using the recombinant nucleoside triphosphate transporter, wherein the unnatural nucleoside triphosphate comprises a base selected from the group consisting of: ### Claim 17 (depends on 16) The cell of claim 16, wherein the unnatural nucleoside comprises a deoxyribose moiety. ### Claim 18 (depends on 16) The cell of claim 16, wherein the unnatural nucleoside comprises a ribose moiety. ### Claim 19 (depends on 12) The cell of claim 12, further comprising an unnatural base pair (UBP) comprising a first unnatural nucleoside and a second unnatural nucleoside, wherein the first unnatural nucleoside comprises a base that is and the second unnatural nucleoside comprises a base selected from the group consisting of ### Claim 20 (depends on 19) The cell of claim 19, wherein the second unnatural nucleoside comprises a base that is ### Claim 21 (depends on 12) The cell of claim 12, wherein the cell is isolated and purified. ### Claim 22 (depends on 1) A nucleic acid molecule encoding the recombinant nucleoside triphosphate transporter of claim 1. ### Claim 23 (depends on 2) A nucleic acid molecule encoding the recombinant nucleoside triphosphate transporter of claim 2. ### Claim 24 (depends on 3) A nucleic acid molecule encoding the recombinant nucleoside triphosphate transporter of claim 3. ### Claim 25 (depends on 4) A nucleic acid molecule encoding the recombinant nucleoside triphosphate transporter of claim 4. ### Claim 26 (depends on 5) A nucleic acid molecule encoding the recombinant nucleoside triphosphate transporter of claim 5. ### Claim 27 (depends on 6) A nucleic acid molecule encoding the recombinant nucleoside triphosphate transporter of claim 6. ### Claim 28 (depends on 7) A nucleic acid molecule encoding the recombinant nucleoside triphosphate transporter of claim 7. ### Claim 29 (depends on 8) A nucleic acid molecule encoding the recombinant nucleoside triphosphate transporter of claim 8. ### Claim 30 (depends on 9) A nucleic acid molecule encoding the recombinant nucleoside triphosphate transporter of claim 9.