Production of carotenoids and apocarotenoids
AssigneeAgency for Science, Technology and Research
InventorsCongqiang Zhang, Xixian Chen, Heng-Phon Too
Life of this patent
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Abstract
A method for producing a carotenoid or apocarotenoid is disclosed. The method comprises the step of expressing in a host cell an expression module comprising an expression vector having a coding region encoding at least one optimised carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter. A host cell comprising an expression vector having a coding region encoding at least one optimised carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter is also provided together with a kit.
Claims21
-53. (canceled)
A method for producing a carotenoid or apocarotenoid comprising the step of expressing in a host cell an expression module comprising an expression vector having a coding region encoding at least one optimized apocarotenoid or carotenoid generating enzyme, the coding region being operably linked to a promoter.
A host cell comprising an expression module comprising expression vector having a coding region encoding at least one optimized apocarotenoid or carotenoid generating enzyme, the coding region being operably linked to a promoter.
A system for producing a carotenoid or apocarotenoid comprising an expression module comprising an expression vector having a coding region encoding at least one optimized carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter, wherein said at least one optimized carotenoid or apocarotenoid generating enzyme is selected from:
- a. ΔN50-LsLcyE and TrxA-Osmanthus fragrans CCD1 for the production of α-ionone;
- or
- b. crtY and phCCD1 for the production of β-ionone;
- or
- c. ΔN50-LsLcyE for the production of ε-carotene;
- or
- d. crtY and blh for the production of retinal;
- or
- e. crtY, blh and ybbO for the production of retinol.
Dependencies are inferred from the claim text.