Methods for in vivo monitoring of dopaminergic disorders and efficacy of treatment agents therefor
AssigneeKenneth L. Rice
InventorKENNETH L. RICE
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Abstract
Provided are methods of evaluating an ability of a therapeutic composition to modulate a dopaminergic activity in an organ of a mammalian subject having a dopaminergic disorder. Also provided are methods of assessing and monitoring the progression of a dopaminergic disorder in tan organ of a mammalian subject, and of optimizing the treatment of such a disorder.
Claims16
A in vivo method for evaluating a therapeutic composition for its ability to modulate a dopaminergic activity in an organ of a mammalian subject having a dopaminergic disorder, comprising:
- administering radiolabeled tropane to the subject;
- determining a baseline level and pattern of binding of the administered radiolabeled tropane to dopamine transporters (DaT) in a the organ of the subject;
- treating the subject with an initial dose of a first therapeutic composition;
- administering a radiolabeled tropane to the treated subject;
- and
- measuring the level and/or pattern of radiolabeled tropane binding to the organ of the treated subject,
- a change in level and/or pattern of radiolabeled tropane binding in the treated subject relative to baseline levels and/or patterns of radiolabeled tropane binding being indicative of the ability of the therapeutic composition to modulate a dopaminergic activity in the organ of the subject.
An in vivo method of obtaining a time course for the progression of a dopaminergic disorder in an organ of a mammalian subject, the method comprising:
- carrying out a detection process, the detection process comprising;
- administering a radiolabeled tropane to the subject;
- and
- acquiring a first tomographic image of the organ;
- and
- repeating the detection process to obtain a second tomographic image of the organ;
- a difference in the second tomographic image relative to the first tomographic image being indicative of a change in the progression of the dopaminergic disorder in the organ of the subject.
An in vivo method of optimizing a treatment regimen of a therapeutic formulation for a dopaminergic disorder in an organ of a mammalian subject, the method comprising:
- administering a radiolabeled tropane to the subject treated with a first dose of the therapeutic formulation;
- measuring a level of tropane binding to the organ by acquiring a tomographic image;
- and
- comparing the level of tropane binding to a projected level of tropane binding obtained from at least one tomographic image acquired before the administering step,
- administering to the subject a second dose of the therapeutic formulation different than the first dose if the level of tropane is different than the projected level of tropane binding.
An in vivo method of determining effectiveness of a neuroprotective agent in a treatment of a mammalian subject having a dopaminergic disorder in an organ, the method comprising:
- administering to the subject treated with the neuroprotective agent a radiolabeled tropane;
- acquiring a tomographic image of the organ via SPECT or PET;
- measuring a level of tropane binding in the organ from the tomographic image;
- and
- determining the effectiveness of the neuroprotective agent based on the level of tropane binding in the organ relative to a projected level of tropane binding, the projected level of binding being obtained from at least one previously acquired tomographic image;
- a difference in the level of tropane binding of at least about 10% being indicative of the effectiveness of the neuroprotective agent.
An in vivo method of detecting binding of a radiolabeled tropane to dopamine transporter (DaT) molecules in the brain of a mammalian subject, the method comprising:
- administering the radiolabeled tropane to a subject;
- initiating the acquisition of a tomographic image about 15 minutes after administering the tropane;
- and
- terminating the tomographic image acquisition about 5 minutes to about 10 minutes after initiation,
- a pattern of tropane binding to DaT molecules in the brain being obtained having two comma-shaped regions that are bilaterally symmetric with each if the brain of the subject is not affected by a dopaminergic disorder of the brain.
Dependencies are inferred from the claim text.