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US 2019/0360003 A1Published application
Production of fatty alcohols in rhodosporidium
AssigneeTemasek Life Sciences Laboratory Limited
InventorsLianghui JI, Yanbin LIU, Chong Mei John KOH, Sihui Amy YAP, Si Te NGOH
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Abstract
The present invention relates to the field of fungal production fatty alcohols. More specifically, the present invention relates to genetically modified host cells, nucleic acid constructs and culture medium for the production of fatty alcohols in Rhodosporidium.
Claims13
A genetically modified fungal cell useful for producing fatty alcohols that comprises:
- (a) a nucleic acid construct that overexpresses a heterologous fatty acyl-CoA reductase (FAR1);
- (b) either (bi) a nucleic acid construct for down-regulating a host cell type 1 acyl-CoA:diacylglycerol acyltransferase (Dga1) or (bii) a knocked-out host cell Dga1 gene or a combination of (bi) and (bii);
- (c) either (ci) a nucleic acid construct for down-regulating a host cell type 2 phospholipid:diacylglycerol acyltransferase (Lro1) or (cii) a knocked-out host cell Lro1 gene or a combination of (ci) and (cii);
- (d) either (di) a nucleic acid construct for down-regulating a host cell acyl-CoA:sterol acyltransferase (steryl ester synthase, Are1) or (dii) a knocked-out host cell Are1 gene or a combination of (di) and (dii);
- and
- (e) either (ei) a nucleic acid construct for down-regulating a host cell type 3 soluble acyltransferase (Dga3) or (eii) a knocked-out host cell Dga3 gene or a combination of (ei) and (eii).
3 more elements
The method of claim 11, wherein the culturing is performed using a medium comprising glucose, yeast extract, K2HPO4, NH4NO3, MgSO4.7H2O, and CaCl2.2H2O.
A culture medium comprising per litre:
- 100 g glucose, 22.5 g yeast extract, 0.75 g K2HPO4, 0.7 g NH4NO3, 0.4 g MgSO4.7H2O, and 0.4 g CaCl2.2H2O.
Dependencies are inferred from the claim text.