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US 2019/0353650 A1Published application
Virus-Like Particle (VLP) Based Small Molecule-Protein Interaction Trap
AssigneesVib Vzw, Universiteit Gent
InventorsJan Tavernier, Sven Eyckerman
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Abstract
This disclosure relates to a virus-like particle in which a small molecule-protein complex is entrapped, ensuring the formation of the small molecule-protein complex under physiological conditions, while protecting the small molecule-protein complex during purification and identification. The disclosure further relates to the use of such virus-like particle for the isolation and identification of small molecule-protein complexes.
Claims19
An artificial virus-like particle (VLP), comprising:
- a VLP-forming polypeptide or a fusion protein comprising a VLP-forming polypeptide;
- a fusion construct comprising first and second small molecules covalently linked to one another, wherein the first small molecule interacts with the VLP-forming polypeptide or fusion protein and the second small molecule interacts with at least one polypeptide other than the VLP-forming polypeptide or fusion protein;
- and
- a polypeptide other than the VLP-forming polypeptide or fusion protein that interacts with the second small molecule.
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A method for detecting a small molecule-protein interaction, the method comprising:
- expressing a virus-like particle (VLP)-forming polypeptide or a fusion protein comprising a VLP-forming polypeptide in a cell;
- recruiting a fusion construct comprising first and second small molecules covalently linked to one another, wherein the first small molecule interacts with the VLP-forming polypeptide or a fusion protein and the second small molecule interacts with at least one polypeptide other than the VLP-forming polypeptide or fusion protein, to said VLP-forming polypeptide;
- interacting a polypeptide other than the VLP-forming polypeptide or fusion protein with the second small molecule to the fusion construct;
- isolating the VLPs;
- and
- analyzing an entrapped complex in an isolated VLP.
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Dependencies are inferred from the claim text.