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US 2019/0352615 A1Published application
Method for purifying virus
AssigneeBlue Sky Vaccines GmbH
InventorsMarkus WOLSCHEK, Manfred REITER
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Abstract
The present invention provides a method for purifying virus particles from host cells infected with virus, wherein the virus particles are treated with benzonase and polyethylene glycol and the use of said purified viruses for therapeutic compositions.
Claims19
A method for purifying virus particles from host cells infected with virus, comprising the following steps in the indicated order:
- a) cultivating said host cells in a cell culture medium under conditions in which the virus particles are released into the cell culture medium,
- b) separating supernatant comprising the virus particles from cell debris and cells by centrifugation,
- c) incubating said supernatant with a nuclease,
- d) precipitating the virus particles with polyethylene glycol (PEG),
- e) centrifuging the virus particles, and
- f) resuspending precipitated virus particles.
3 more elements
Dependencies are inferred from the claim text.