Multiplexed Genome Editing
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Luhan Yang, Marc Guell
Life of this patent
Estimating the term…
Abstract
A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.
Claims25
-43. (canceled)
A genetically engineered porcine cell in which at least 97% of the PERV pol genes comprise a genetically engineered genomic indel.
An isolated porcine organ comprising a plurality of genetically engineered porcine cells, wherein each genetically engineered porcine cell of said plurality is characterized in that at least 97% of the PERV pol genes comprise a genetically engineered genomic indel.
A method of generating a porcine cell substantially free of porcine endogenous retrovirus (PERV), the method comprising inserting one or more genetically engineered genomic indels into at least 97% of the PERV pol genes in the porcine cell, thereby inactivating at least 97% of the PERV pol gene.
Dependencies are inferred from the claim text.