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US 2019/0338324 A1Published application
Process for producing l-methionine from methional
AssigneeEvonik Degussa GmbH
InventorsArne Skerra, Lukas Eisoldt
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Abstract
A method is useful for the biocatalytic synthesis of proteinogenic L-amino acids, such as L-alanine, L-valine. L-methionine. L-leucine, L-isoleucine or L-phenylalanine from a respective aldehyde and carbon dioxide. In particular, the method is useful for the biocatalytic synthesis of L-methionine from 3-methylthio-propanal (“methional”) and carbon dioxide.
Claims11
1:
- A method for producing an L-amino acid, comprising:
- reacting a mixture comprising an aldehyde, carbon dioxide, a decarboxylase, a corresponding decarboxylase cofactor, and
- (a) at least one donor amino acid and an aminotransferase, and/or
- (b) NADH, ammonia and/or an ammonium salt and an amino acid dehydrogenase,
- to form said L-amino acid or a salt thereof.
2 more elements
2:
- The method as claimed in claim 1, wherein the L-amino acid to be formed is at least one member selected from the group consisting of L-alanine, L-valine, L-methionine, L-leucine, L-isoleucine and L-phenylalanine.
3:
- The method as claimed in claim 1, wherein the L-amino acid to be formed is L-methionine and the aldehyde is 3-(methylthio)-propanal (methional).
4:
- The method as claimed in claim 1, wherein the corresponding decarboxylase cofactor comprises thiamine pyrophosphate.
5:
- The method as claimed in claim 1, wherein the decarboxylase is at least one member selected from the group consisting of pyruvate decarboxylase PDC1, which originates from Saccharomyces cerevisiae, phenylpyruvate decarboxylase ARO10, which originates from Saccharomyces cerevisiae, and branched chain decarboxylase KdcA, which originates from Lactococcus lactis, as well as mutants and variants of these decarboxylases having decarboxylase activity.
6:
- The method as claimed in claim 1, wherein the aminotransferase is present, and is at least one member selected from the group consisting of methionine aminotransferase YbdL, which originates from E. coli, and aromatic aminotransferase Aro8, which originates from Saccharomyces cerevisiae, as well as mutants and variants of these aminotransferases having aminotransferase activity.
7:
- The method as claimed in claim 1, wherein the amino acid dehydrogenase is present, and is at least one member selected from the group consisting of leucine dehydrogenase LeuDH, which originates from Bacillus sphaericus, and phenylalanine dehydrogenase PheDH, which originates from Thermoactinomyces intermedius, as well as well as mutants and variants of these amino acid dehydrogenases having amino acid dehydrogenase activity.
8:
- The method as claimed in claim 1, wherein the donor amino acid is present, different from the L-amino acid to be formed, and is at least one L-amino acid selected from the group consisting of L-glutamine, L-glutamate, L-alanine, L-phenylalanine, L-tyrosine, L-leucine, L-isoleucine, L-histidine and L-tryptophan.
9:
- The method as claimed in claim 1, wherein the carbon dioxide is applied to the mixture at a pressure from 10 to 7400 kPa.
10:
- The method as claimed in claim 1, wherein the mixture further comprises formic acid or a salt thereof and a formate dehydrogenase.
11:
- The method as claimed in claim 10, wherein the formate dehydrogenase is at least one member selected from the group consisting of formate dehydrogenase from Pseudomonas sp. and formate dehydrogenase from Candida sp. as well as mutants and variants of these formate dehydrogenases having formate dehydrogenase activity.