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US 2019/0338259 A1Published application
Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation
AssigneeZymergen Inc.
InventorsCara Ann Tracewell, Steven M. Edgar, Stepan Tymoshenko, Alexander Glennon Shearer
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Abstract
The present disclosure describes the engineering of microbial cells for fermentative production of (6E)-8-hydroxygeraniol and provides novel engineered microbial cells and cultures, as well as related (6E)-8-hydroxygeraniol production method.
Claims20
An engineered microbial cell, wherein the engineered microbial cell expresses:
- (a) a non-native geranyl diphosphate diphosphatase (geraniol synthase);
- and
- (b) a non-native geraniol-8-hydroxylase;
- wherein the engineered microbial cell comprises increased activity of one or more upstream (6E)-8-hydroxygeraniol pathway enzyme(s), said increased activity being increased relative to a control cell, the one or more upstream pathway enzymes being selected from the group consisting of ATP-citrate synthase, an acetyl-CoA synthetase, a thiolase, a hydroxymethylglutaryl coenzyme A synthase (HMG-CoA synthase), a hydroxymethylglutaryl coenzyme A reductase (HMG-CoA reductase), a mevalonate kinase, a phosphomevalonate kinase, a diphosphomevalonate decarboxylase, an isopentenyl-diphosphate delta-isomerase, and a geranyl diphosphate synthase;
- and
- wherein the engineered microbial cell produces (6E)-8-hydroxygeraniol.
3 more elements
An engineered microbial cell, wherein the engineered microbial cell comprises:
- (a) means for expressing a non-native native geranyl diphosphate diphosphatase (geraniol synthase);
- and
- (b) means for expressing a non-native geraniol-8-hydroxylase;
- wherein the engineered microbial cell comprises increased activity of one or more upstream (6E)-8-hydroxygeraniol pathway enzyme(s), said increased activity being increased relative to a control cell, the one or more upstream pathway enzymes being selected from the group consisting of ATP-citrate synthase, an acetyl-CoA synthetase, a thiolase, a hydroxymethylglutaryl coenzyme A synthase (HMG-CoA synthase), a hydroxymethylglutaryl coenzyme A reductase (HMG-CoA reductase), a mevalonate kinase, a phosphomevalonate kinase, a diphosphomevalonate decarboxylase, an isopentenyl-diphosphate delta-isomerase, and a geranyl diphosphate synthase;
- and
- wherein the engineered microbial cell produces (6E)-8-hydroxygeraniol.
3 more elements
Dependencies are inferred from the claim text.