Multi-Specific Molecules

A multi-specific molecule capable of binding to two or more different target antigens or epitopes, the molecule comprising:

• at least one binding domain molecule (BDM) which binds to a first target antigen or epitope, the BDM comprising a V-like domain (VLD) scaffold which comprises an extracellular portion of human CTLA-4 in soluble monomeric form and having three exposed binding loops (BLs) contained within and wherein at least one of the three BLs are modified or replaced relative to their corresponding native sequence in the scaffold for selective binding to a heterologous target antigen or epitope;
• and
• (ii) a pharmacologically active protein or peptide which is an antibody or antigen-biding fragment thereof or a non-antibody protein or peptide which binds to a second target antigen or epitope;
• wherein at least one BDM is coupled to a C-terminus of each heavy and/or light chain of the antibody or antigen binding fragment thereof, or is coupled to a C-terminus of each polypeptide chain of the non-antibody protein or peptide.