Materials and methods for differential biosynthesis in species of the genera ralstonia and cupriavidus and organisms related thereto
AssigneeInvista North America S.A.R.L.
InventorsAlexander Brett FOSTER, Ana Teresa dos Santos Brito Mendes ROBERTS, Arghya BARMAN, Jonathan KENNEDY
Life of this patent
Estimating the term…
Abstract
Methods for increasing carbon-based chemical product yield in an organism by increasing carbon uptake and/or altering a pathway to or from an overflow metabolite in the organism, nonnaturally occurring organisms having increased carbon-based chemical product yield with increased carbon uptake and/or an altered pathway to or from an overflow metabolite, and methods for producing a carbon-based chemical product with these organisms are provided.
Claims16
A method for increasing carbon-based chemical product yield in an organism, said method comprising modifying an organism selected from a species of Cupriavidus or Ralstonia with diminished polyhydroxybutyrate synthesis or an organism with properties similar thereto by modulating activity of one or more polypeptides or functional fragments thereof functioning to increase carbon uptake and/or in a pathway to or from an overflow metabolite, thereby increasing carbon-based chemical product yield in the organism as compared to an organism without said modulated polypeptide activity.
A nonnaturally occurring organism capable of yielding a carbon-based chemical product, said organism selected from a species of Cupriavidus or Ralstonia with diminished polyhydroxybutyrate synthesis or an organism with properties similar thereto and modified to increase carbon uptake and/or alter a pathway to or from an overflow metabolite by modulating activity of one or more polypeptides or functional fragments thereof functioning to increase carbon uptake and/or in a pathway to or from an overflow metabolite.
Dependencies are inferred from the claim text.