Compositions and methods for exosome targeted expression
AssigneeMorehouse School of Medicine
InventorsVincent C. Bond, Michael Powell, Ming Bo Huang, Syed Ali
Life of this patent
Estimating the term…
Abstract
The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.
Claims22
A method for preparing a protein composition, comprising:
- a) culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest;
- (b) isolating exosomes from cultured exosome-producing cell;
- and
- (c) purifying said protein of interest from isolated exosomes.
A cell line that produces exosomes containing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest, wherein said Nef-derived peptide comprises SEQ ID NO:3, 4, 5 or 6.
A method for delivering a protein of interest to a target cell in a mammal, comprising administering to the mammal an exosome comprising a Nef-fusion protein, wherein said Nef-fusion protein comprises a Nef-derived peptide fused to said protein of interest and wherein said Nef-derived peptide comprises SEQ ID NO:3, 4, 5 or 6.
A method for inducing an immune response in a mammal, comprising administering to a mammal an exosome comprising a Nef-fusion protein comprising a Nef-derived peptide fused to an immunogenic protein of interest, wherein said Nef-derived peptide comprises SEQ ID NO:3, 4, 5 or 6.
A method for detecting a target molecule in a sample, comprising:
- contacting a sample from a subject with a Nef-fusion protein that binds specifically to said target molecule, wherein said Nef-fusion protein comprises SEQ ID NO:3, 4, 5 or 6.
- detecting a binding of said target molecule in the sample to said Nef-fusion protein, and
- determining a level of said target molecule in said sample,
- wherein a medical condition is indicated if a level of said target molecule is outside a reference range.
A pharmaceutical composition, comprising:
- an exosome comprising a Nef-fusion protein containing a Nef-derived peptide fused to a protein of interest;
- and
- a pharmaceutically acceptable carrier.
A Nef-fusion protein, produced by
- culturing cells that produce exosomes containing said Nef-fusion protein;
- isolating exosomes from said cells;
- and purifying said Nef-fusion protein from isolated exosomes, wherein said Nef-fusion protein comprises a Nef-derived peptide fused to a protein of interest and wherein said Nef-derived peptide comprises SEQ ID NO:3, 4, 5 or 6.
Dependencies are inferred from the claim text.