Compositions and methods for exosome targeted expression
AssigneeMorehouse School of Medicine
InventorsVincent C. BOND, Michael Powell, Ming Bo Huang, Syed Ali
Life of this patent
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Abstract
The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.
Claims12
1-9. (canceled)
A method for delivering a protein of interest to a target cell in a mammal, comprising administering to the mammal an exosome comprising a Nef-fusion protein, wherein said Nef-fusion protein comprises a Nef-derived peptide fused to said protein of interest and wherein said Nef-derived peptide comprises SEQ ID NO:3, 4, 5 or 6.
16-22. (canceled)
Dependencies are inferred from the claim text.