Cell penetrating peptides with improved internalization properties
AssigneesSorbonne Universite, Inserm (Institut National De La Sante Et De La Recherche Medicale), Centre National De La Recherche Scientifique, Pep-Therapy
InventorsANGELITA REBOLLO, JESUS MARIA FOMINAYA GUTIERREZ, JERONIMO BRAVO SICILIA, SOPHIE LEBEL BINAY, LETICIA DOMINGUEZ BERROCAL
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Abstract
The invention relates to a method for delivering a cargo molecule into a cell, which method comprises linking said cargo molecule to a cell penetrating peptide. The chimeric constructs comprising such cell penetrating moieties are encompassed.
Claims21
1-20. (canceled)
A construct comprising:
- a) a cell penetrating moiety consisting of KKKKKWKKWKKK (SEQ ID NO:
- 7) or KKKKKWKKW (SEQ ID NO:
- 9), linked to a cargo molecule;
- or
- b) a chimeric polypeptide comprising i) a cell penetrating moiety consisting of RRWRRWRRWRR (SEQ ID NO:
- 6) or RRWRRWRRW (SEQ ID NO:
- 8), fused to ii) a cargo molecule that is a peptide.
A construct comprising i) sequence RKR-KKKKKWKKW-PKKKKLD (SEQ ID NO:
- 27) or RKR-KKKKKWKKWKKK-PKKKKLD (SEQ ID NO:
- 28), fused to ii) a pro-apoptotic peptide.
A (poly)peptide comprising a sequence selected from the group consisting of KKKKKWKKWKKK (SEQ ID NO:
- 7);
- KKKKKWKKW (SEQ ID NO:
- 9);
- RKR-KKKKKWKKW-PKKKKLD (SEQ ID NO:
- 27) and RKR-KKKKKWKKWKKK-PKKKKLD (SEQ ID NO:
- 28).
Dependencies are inferred from the claim text.