Peptoid affinity ligands
AssigneeMike-Ann, LLC
InventorsAndrew J. Murphy, Tee Bordelon, Michael Crapanzano
Life of this patent
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Abstract
Disclosed herein are peptoids and related compounds, including peptoid affinity ligands for binding and/or purifying immunoglobulins, immunoglobulin fragments or immunoglobulin fusion proteins thereof. Methods of making peptoid affinity ligands and using the same to bind, purify and/or isolate immunoglobulins and related compounds are also disclosed. Such peptoid affinity ligands comprise a peptoid compound consisting of sequentially coupled peptoid residues forming a peptoid backbone, with one or more functional groups appended to a nitrogen of the peptoid residues of the peptoid backbone configured to provide the desired binding affinity.
Claims29
A peptoid affinity ligand, the peptoid affinity ligand comprising:
- a peptoid compound consisting of sequentially coupled peptoid residues forming a peptoid backbone, with one or more functional groups appended to a Nitrogen of the peptoid residues of the peptoid backbone;
- wherein the one or more functional groups comprise, in any order but coupled to sequential peptoid residues on the peptoid backbone:
- at least two aromatic functional groups and either a basic or acidic functional group;
- subject to the proviso that excluded therefrom are peptoid affinity ligands containing a contiguous segment of three peptoid residues with the following functional groups:
- (i) a basic residue, (ii) an aromatic residue, and (iii) a basic residue or hydrophilic residue;
- and
- wherein the peptoid affinity ligand specifically binds an immunoglobulin, immunoglobulin fragment or immunoglobulin fusion protein thereof, wherein the immunoglobulin, immunoglobulin fragment or immunoglobulin fusion protein thereof is one or more of IgG, IgA, IgE, IgD, IgM or IgY.
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A peptoid affinity ligand, the peptoid affinity ligand comprising:
- a peptoid compound consisting of sequentially coupled peptoid residues forming a peptoid backbone, with one or more functional groups appended to a Nitrogen of the peptoid residues of the peptoid backbone;
- wherein the one or more functional groups include at least two aromatic functional groups and at least one basic or acidic functional group;
- subject to the proviso that excluded therefrom are peptoid affinity ligands containing, from a N-terminus to a C-terminus of the peptoid backbone, a contiguous segment of three peptoid residues with the following functional groups:
- (i) a basic residue, (ii) an aromatic residue, and (iii) a basic residue or hydrophilic residue;
- wherein the peptoid affinity ligand specifically binds an immunoglobulin, immunoglobulin fragment or immunoglobulin fusion protein thereof, wherein the immunoglobulin, immunoglobulin fragment or immunoglobulin fusion protein thereof is one or more of IgG, IgA, IgE, IgD, IgM or IgY.
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A peptoid affinity ligand selected from the group consisting of:
- wherein the peptoid affinity ligand has a binding affinity to an immunoglobulin, immunoglobulin fragment or immunoglobulin fusion protein thereof.
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Dependencies are inferred from the claim text.