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US 2019/0336969 A1Published application
Parallelized sample handling
AssigneesCalifornia Institute of Technology, The University of Chicago
InventorsRustem F. ISMAGILOV, Liang MA, Qichao PAN, Mikhail KARYMOV, Toan HUYNH, George SAWICKI, Stefano BEGOLO, Wenbin DU
Life of this patent
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Abstract
Provided herein are methods, compositions, and devices for the parallel handling of samples, such as cells or other biological samples. The methods, compositions, and devices are suited for multiple levels of analysis, including genetic and functional assays, of samples.
Claims29
-17. (canceled)
A method, comprising:
- (a) providing a first substrate comprising a first defined volume;
- (b) providing a second substrate comprising second defined volume, said second substrate coupled to said first substrate;
- (c) loading a first sample into said first defined volume;
- (d) bringing said first defined volume into fluidic contact with said second defined volume, wherein said first substrate remains coupled to said second substrate;
- (e) mixing contents of said first defined volume with contents of said second defined volume, thereby producing a mixed sample;
- (f) separating said first defined volume from said second defined volume, wherein a first part of said mixed sample is contained by said first defined volume and a second part of said mixed sample is contained by said second defined volume, wherein said first substrate remains coupled to said second substrate;
- and
- (g) decoupling said first substrate from said second substrate, wherein said first part of said mixed sample remains contained by said first defined volume and said second part of said mixed sample remains contained by said second defined volume.
5 more elements
-35. (canceled)
A method, comprising:
- (a) dispersing a sample among a plurality of defined volumes;
- (b) splitting said plurality of defined volumes, essentially simultaneously, into a plurality of matched pairs of daughter volumes comprising a plurality of first daughter volumes and a plurality of matched second daughter volumes, wherein said splitting is performed without the application of a pumping force to said defined volumes;
- (c) conducting at least one analysis on said plurality of said first daughter volumes;
- and
- (d) selecting a subset of said plurality of matched second daughter volumes based on said analysis.
2 more elements
(canceled)
-41. (canceled)
-60. (canceled)
A method, comprising:
- (a) providing a plurality of defined volumes, each of said plurality of defined volumes comprising one of a plurality of samples;
- (b) subjecting said plurality of samples to a set of conditions;
- (c) conducting a process on said plurality of samples;
- (d) transferring said plurality of samples from said plurality of defined volumes to a shared container, thereby creating a pooled sample;
- (e) conducting an analysis on said pooled sample;
- and
- (f) determining from said analysis the extent to which said set of conditions enabled or did not enable said process.
4 more elements
(canceled)
(canceled)
-68. (canceled)
-77. (canceled)
Dependencies are inferred from the claim text.