Dot Blot Box and Use Thereof
AssigneeUniversity of South Carolina
InventorsLAUREN M. WOLF, MELISSA A. MOSS
Life of this patent
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Abstract
Devices (dot blot boxes) for use in immunoblotting are described. The dot blot boxes can include a series of hooks, clips or the like on opposite sides of a container for retaining a test strip there between. The dot blot boxes can be sized to retain a plurality of test strips generally parallel to one another such that they do not contact one another and do not contact the base of the container. A container can hold a fluid, e.g., a blocking buffer solution or antibody solution, and the test strips can be submerged in the fluid while retained in the container.
Claims16
A dot blot box comprising:
- a container comprising a first wall having a first surface and a second wall having a second surface, the first and second surfaces facing one another across a base of the container;
- a first holder located on the first surface;
- and
- a second holder located on the second surface, the first and second holders being configured to retain first and second ends, respectively, of a test strip.
The dot blot box of claim 1, wherein the holders comprise a plastic or a metal. The dot blot box of claim 6, wherein the metal comprises aluminum.
A method for carrying out a protein blocking protocol, comprising:
- contacting a test strip with a test sample, the test sample comprising a protein;
- retaining a first end of the test strip at a first holder, the first holder being located at a first wall of a dot blot box;
- retaining a second end of the test strip at a second holder, the second holder being located at a second wall of the dot blot box, the second wall facing the first wall;
- and
- submerging the test sample held on the test strip in a solution retained in the container.
Dependencies are inferred from the claim text.