Process for purifying nadph
AssigneesBontac Bio-Engineering (Shenzhen) Co., Ltd, Jiangxi Bontac Green-Biocatalysis Ecoindustrial Park Co.,Ltd
InventorsQi ZHANG, Dongmin ZHANG, Rongzhao FU
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Abstract
The present invention relates to a process for purifying crude NADPH product prepared by biocatalysis. The objective of the present invention is to solve the technical problems of low yield and low purity of the purified product in the existing ion exchange resin method. The present invention comprises sequentially the following steps: pretreatment, loading onto an ion column, elution of cations, pre-elution of impurities etc. The yield of the purification process disclosed in the present invention can be up to 85% or higher and the purified NADPH has a purity of up to 98% or higher.
Claims17
A process for purifying NADPH, comprising the following steps:
- A. pretreatment:
- filtering a crude NADPH product prepared by biocatalysis through a microfiltration membrane to obtain a microfiltered crude NADPH product, wherein the microfiltration membrane is a hollow fiber membrane having a pore size of 0.1-0.5 μm;
- B. loading onto an ion column:
- loading the microfiltered crude NADPH product obtained in Step A onto an anion exchange resin column;
- C. elution of cations:
- after loading, washing the ion column with purified water until no cations are contained in the eluate;
- D. pre-elution of impurities:
- washing the ion column with a 0.07-0.5 mol/L sodium chloride solution until no substances having UV absorption at 260 nm are contained in the eluate;
- E. elution of product:
- washing the ion column with an aqueous solution containing 0.5 mol/L sodium chloride and 7% (V/V) ethanol, and collecting an eluate haying UV absorption at 340 nm;
- and
- F. concentrating and drying the eluate obtained in Step E to obtain a purified NADPH product.
Dependencies are inferred from the claim text.