Systems, methods and compositions for optical stimulation of target cells
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsKarl Deisseroth, Feng Zhang, Viviana Gradinaru
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Abstract
Methods, systems and devices are implemented in connection with light-responsive ion channel molecules. One such method is implemented using a light-activated ion channel molecule that responds to a light stimulus. The method includes engineering the light-activated ion channel molecule in a cell; and activating the ion channel molecule, in response to light stimulus that is provided to the ion channel molecule and that has properties that do not activate a ChR2 ion channel, to allow ions to pass through the light-activated ion channel molecule.
Claims22
-29. (canceled)
A method for separably modulating activity of a first cell population and a second cell population, the method comprising:
- activating a Volvox carteri light-responsive ion channel (VChR1) protein with yellow light, wherein the first cell population is engineered to express the VChR1 protein;
- and
- activating a Chlamydomonas reinhardtii light-responsive (ChR2) protein with blue light, wherein the second cell population is engineered to express the ChR2 protein.
A treatment method comprising:
- a) implanting into an individual a device comprising:
- i) a first recombinant expression vector comprising a nucleotide sequence encoding a Volvox carteri light-responsive ion channel (VChR1) protein;
- ii) a second recombinant expression vector comprising a nucleotide sequence encoding a Chlamydomonas reinhardtii light-responsive (ChR2) protein;
- and
- iii) a light-delivery mechanism,
- wherein the VChR1 protein is produced in a first cell population in the individual, and the ChR2 protein is produced in a second cell population in the individual;
- and
- b) separably activating the VChR1 protein and the ChR2 protein with yellow light and blue light, respectively, from the light-delivery mechanism, thereby providing a treatment to the individual.
Dependencies are inferred from the claim text.