US 2019/0336551 A1 · Herpesvirus with Modified Glycoprotein B

Abstract

The present invention is directed to a recombinant herpesvirus comprising a heterologous polypeptide ligand capable of binding to a target molecule and fused to or inserted into glycoprotein B at specific sites. The herpesvirus may comprise more than one ligand, and the additional ligand(s) may be comprised by a modified glycoprotein D and/or modified glycoprotein H. This allows the herpesvirus to target a cell for therapeutic purposes, and a cell for virus production. The present invention further comprises a pharmaceutical composition comprising the herpesvirus, the herpesvirus for use in the treatment of a tumor, infection, degenerative disorder or senescence-associated disease, a nucleic acid and a vector coding for the gB, a polypeptide comprising the gB, and a cell comprising the herpesvirus, nucleic acid, vector or polypeptide. Moreover, a method for infecting a cell with the herpesvirus or for producing the herpesvirus is disclosed.

Claims16

Dependent claims

A recombinant herpesvirus comprising a heterologous polypeptide ligand capable of binding to a target molecule and fused to or inserted into glycoprotein B (gB) present in the envelope of the herpesvirus,

wherein the ligand is fused to gB, or
wherein the ligand is inserted at any amino acid within a disordered region of gB, but is not inserted at any amino acid within the region spanning from amino acids 77 to 88 of gB according to SEQ ID NO:
1 or within a corresponding region of a homologous gB, or
wherein the ligand is inserted at any amino acid within a region spanning from amino acids 31 to 77 or 88 to 184, preferably amino acids 31 to 77 or 88 to 136 or more preferably 31 to 77 or 88 to 108, and/or within a region spanning from amino acids 409 to 545, preferably amino acids 459 to 545, more preferably amino acids 459 to 497, or still more preferably amino acid 460 to 491, of gB according to SEQ ID NO:
1 or within a corresponding region of a homologous gB, or
wherein the ligand has a length of 5 to 120 amino acids and is inserted at any amino acid within a region spanning from amino acids 77 to 88 of gB according to SEQ ID NO:
1 or within a corresponding region of a homologous gB.

4 more elements

Dependencies are inferred from the claim text.

Herpesvirus with Modified Glycoprotein B

Life of this patent

Herpesvirus with Modified Glycoprotein B

Life of this patent

Abstract

Claims16