Synthetic bacteria and methods of use

A synthetic bacteria derived from a non-pathogenic bacteria, the synthetic bacteria comprising:

• a) a porphyrin pathway comprising a biomolecule introduced, removed, or modified relative to the porphyrin pathway of the non-pathogenic bacteria;
• provided that porphyrin is not produced by the synthetic bacteria or is produced at a level less than about 4 micromolar of porphyrin in the synthetic bacteria;
• b) a modified lipase compared to a native lipase;
• c) a biomolecule introduced, wherein the biomolecule is adapted to produce an enzyme at a tunable level to effect a second metabolic pathway of the synthetic bacteria;
• d) a vitamin B12 metabolic pathway comprising a biomolecule introduced, removed, or modified relative to the vitamin B12 metabolic pathway of the non-pathogenic bacteria;
• provided that the synthetic bacteria produces a high levels of intracellular vitamin B12 as compared to the non-pathogenic bacteria;
• e) a hyaluronidase from a heterologous species as the non-pathogenic bacteria;
• f) a nitrous oxide pathway comprising a biomolecule introduced, removed, or modified relative to the nitrous oxide pathway of the non-pathogenic bacteria;
• provided that the synthetic bacteria produces nitrous oxide;
• g) a fatty acid synthesis pathway comprising a biomolecule introduced, removed, or modified relative to the fatty acid synthesis pathway of the non-pathogenic bacteria;
• provided that the synthetic bacteria produces fatty acids;
• h) a biomolecule introduced, removed, or modified relative to the citric acid pathway of the non-pathogenic bacteria;
• provided that the synthetic bacteria produces glycine;
• i) a biomolecule introduced, wherein the biomolecule is adapted to secrete a TNF-alpha inhibitor, an interleukin-8 inhibitor, a tumor neutrophil chemotaxis inhibitor, an interleukin-6 inhibitor, an NFkB inhibitor, human beta defensing-1 inhibitor, human beta defensing-2 inhibitor, or a human beta defensing-3 inhibitor;
• j) a biomolecule introduced, wherein the biomolecule is adapted to secrete a corticotropin releasing hormone (CRH), a corticotropin releasing hormone receptor (CRHR), a corticotropin releasing hormone binding protein (CRHBP), or a combination thereof;
• or
• k) any combination of (a), (b), (c), (d), (e), (f), (g), (h), (i) and (j).