Artificial Cells and Delivery Devices for Use in Tissue Engineering, and Related Methods
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsMorgan Virginia Fedorchak, Jeffrey Krawiec, Steven R. Little, Katherine Lorentz, David A. Vorp, Justin Weinbaum
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Abstract
Provided herein is a drug delivery device and composition, such as a particle, comprising conditioned medium. Also provided herein is a method of preparing polymeric particles for release of conditioned medium. Further, a tissue growth scaffold comprising particles for release of conditioned medium is provided.
Claims21
A composition comprising a first particle comprising conditioned medium from a cell culture within the particle, and having a first release profile of the conditioned medium, optionally in blood, water, PBS or saline.
A controlled release pharmaceutical device comprising conditioned medium.
A method of preparing a controlled-release composition comprising:
- a. culturing cells in cell culture medium, thereby producing conditioned medium;
- b. collecting the conditioned medium;
- c. optionally concentrating the conditioned medium;
- d. emulsifying, for example by sonicating, the medium in a volatile solvent, comprising a polymer to produce a micro-emulsion;
- and
- e. homogenizing the micro-emulsion in an aqueous phase, so that particles precipitate as the solvent evaporates.
Dependencies are inferred from the claim text.