Lipolytic enzyme variants
AssigneeDsm IP Assets B.V.
InventorsRene Marcel DE JONG, Chantal CHRISTIS, Neil CARR
Life of this patent
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Abstract
The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 53, 112, 141, 178, 179, 182, 202, 203, 235, 282, 284, said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2. Such a variant polypeptide may be used in the preparation of a baked product.
Claims16
A variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO:
- 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 53, 112, 141, 178, 179, 182, 202, 203, 235, 282, 284, said positions being defined with reference to SEQ ID NO:
- 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO:
- 2.
A method of producing a lipolytic polypeptide variant, which method comprises:
- a) selecting a polypeptide having lipolytic activity;
- b) substituting at least one amino acid residue at a position corresponding to any of the positions 53, 112, 141, 178, 179, 182, 202, 203, 235, 282, 284,
- said positions being defined with reference to SEQ ID NO:
- 2;
- c) optionally substituting one or more further amino acids as defined in b);
- d) preparing the variant resulting from a)-c);
- e) determining a property of the variant;
- and
- f) selecting a variant having an altered property in comparison with a reference polypeptide having lipolytic activity, thereby to produce a lipolytic polypeptide variant, wherein the altered property includes an increased ratio of the activity on esters of long chain fatty acids:
- the activity on esters of short chain fatty acids as compared to the reference polypeptide having lipolytic activity measured under the same conditions.
Dependencies are inferred from the claim text.