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AssigneesApplied Stemcell, Inc., The Regents of the University of Michigan
InventorsRuby Yanru TSAI, Jie XU, Yuqing CHEN, Jifeng ZHANG, Ling-jie KONG
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Provided is a genetically modified non-human mammal that comprises an anchor DNA sequence inserted at an endogenous locus of a secretory milk protein gene, wherein the anchor DNA sequence comprises a site-specific recombinase recognition site. Also provided is a genetically modified non-human mammal that comprises a transgene inserted at an endogenous locus of a secretory milk protein gene, wherein the transgene encodes a secretory protein and is operably linked to the endogenous promoter of said secretory milk protein gene, and wherein the transgene is flanked by a pair of site-specific recombinase resulting sites. The genetically modified non-human mammals provided can be used for producing the secreted recombinant protein encoded by the transgene from the milk produced by the genetically modified non-human mammals.
A genetically modified non-human mammal comprising an anchor DNA sequence inserted at an endogenous locus of a secretory milk protein gene, wherein said anchor sequence comprises a first site-specific recombinase recognition site.
A genetically modified non-human mammal comprising a transgene inserted at an endogenous locus of a secretory milk protein gene, wherein said transgene encodes a secretory protein and is operably linked to the endogenous promoter of said secretory milk protein gene, and wherein said transgene is flanked by a first site-specific recombinase resulting site and a second site-specific recombinase resulting site.
The non-human mammal of claim of 11, wherein the transgene comprises a polyA sequence at its 3′ end.
The non-human mammal of claim of 11, wherein the transgene encodes a therapeutic protein selected from the group consisting of antigen binding proteins, antibodies, vaccines, fusion proteins, enzymes, co-enzymes, and lysosomal proteins.
A method for making a genetically modified non-human mammal, comprising
A method for making a genetically modified non-human mammal, comprising
Dependencies are inferred from the claim text.