Reagents and methods for detecting protein lysine 3-hydroxybutyrylation
AssigneePtm Bio LLC
InventorsYingming ZHAO, Zhongyu XIE
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Abstract
The invention provides an isolated peptide comprising a lysine 3-hydroxybutyrylation site, a lysine 3-hydroxybutyrylation specific affinity reagent that specifically binds to the peptide, and a method for detecting protein lysine 3-hydroxybutyrylation in a sample using the reagent.
Claims20
A method for producing an isolated affinity reagent that binds specifically to a protein or a fragment thereof comprising a lysine when the lysine is 3-hydroxyisobutyrylated, wherein the isolated affinity reagent does not bind to the protein or fragment thereof when the lysine is not 3-hydroxyisobutyrylated, comprising immunizing a host with a peptide comprising a 3-hydroxyisobutyrylated lysine.
A method for producing an isolated affinity reagent that binds specifically to a protein or a fragment thereof comprising a lysine when the lysine is 3-hydroxyisobutyrylated, wherein the isolated affinity reagent does not bind to the protein or fragment thereof when the lysine is not 3-hydroxyisobutyrylated, comprising screening a protein library using a peptide comprising a 3-hydroxyisobutyrylated lysine.
Dependencies are inferred from the claim text.