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US 2019/0330594 A1Published application
Method for preparing composition, included within exosome obtained from nanog-introduced, fetus-derived mesenchymal stem cell in amniotic fluid, for hair growth
AssigneeStemlab Inc.
InventorsSeung Kwon YOU, Jung-Hyun PARK, Ji-Hoon JANG, Eun Kyoung JUN
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Abstract
A composition for hair growth and a preparation method therefor through the separation of exosomes from a culture medium of fetus-derived mesenchymal stem cells overexpressing the dedifferentiation factor Nanog in amniotic fluid are described.
Claims5
A method of producing a mesenchymal stem cell-derived exosome, the method comprising:
- (a) isolating a fetus-derived cell from amniotic fluid obtained from a pregnant woman;
- (b) sub-culturing the isolated fetus-derived cell in a medium containing fetal bovine serum (FBS) and basic fibroblast growth factor (bFGF) to obtain a mesenchymal stem cell derived from a fetus in amniotic fluid;
- (c) introducing Nanog into the mesenchymal stem cell derived from a fetus in amniotic fluid to obtain a Nanog-overexpressing mesenchymal stem cell derived from a fetus in amniotic fluid;
- (d) culturing the obtained Nanog-overexpressing mesenchymal stem cell derived from a fetus in amniotic fluid for 1 day to 5 days to prepare a conditioned medium;
- and
- (e) isolating an exosome from the conditioned medium,
- wherein the isolated exosome comprises any one or more human growth factors selected from the group consisting of basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF), Wingless-type MMTV integration site family member 7A (Wnt7a), and platelet-derived growth factor (PDGF)-AA.
4 more elements
Dependencies are inferred from the claim text.