Self-assembling insect ferritin nanoparticles for display of co-assembled trimeric antigens

A recombinant insect ferritin nanoparticle, comprising:

• twelve recombinant insect ferritin heavy chain fusion proteins and twelve recombinant insect ferritin light chain fusion proteins self-assembled into a globular nanoparticle;
• and
• eight self-assembled heterologous trimeric antigens extending radially outward from the outer surface of the globular nanoparticle, the eight heterologous trimeric antigens comprising four trimers of a first protein and four trimers of a second protein having a different amino acid sequence from the first protein;
• wherein
• the recombinant insect ferritin nanoparticle comprises a shape having a tetrahedral symmetry;
• the recombinant insect ferritin heavy chain fusion proteins comprise an N-terminal fusion of the first protein to a recombinant insect ferritin heavy chain;
• and
• the recombinant insect ferritin light chain fusion proteins comprise an N-terminal fusion of the second protein to a recombinant insect ferritin light chain.