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US 2019/0329154 A1Published application
Hybrid supercritical fluid chromatography method
AssigneePIC Solution
InventorMohamed SHAIMI
Life of this patent
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Abstract
The invention relates to a method of supercritical fluide or liquid chromatography of a product P. The method according to the invention comprises, continuously in this order: an analytical step (10, 30) according to an analytical route (1); a switching step (X1, X2) comprising a route changing operation (402, 502) between the analytical route (1) and a preparative route (2); if the preparative route (2) is selected in the switching step, a preparative step (20) according to the preparative route (2).
Claims15
A method of supercritical fluid or liquid chromatography of a product P, said method comprising continuously, in this order:
- an analytical step according to an analytical route comprising:
- an operation of first pumping of a supercritical fluid or liquid and second pumping of a modifier,
- an operation of injection of at least one sample of the product P or of at least one constituent of the product P into at least one chromatography column, the product P or the constituent of the product P being eluted with the supercritical fluid or liquid and the modifier,
- an operation of separation of the constituents of the product P,
- an operation for detection of the composition of the product P or of the constituents of the product P,
- a switching step comprising
- a verification operation comprising a substep of comparing the analysis results with one another or with reference values representing optimum analysis conditions, and
- an operation of automatic route changing between the analytical route and a preparative route as a function of the preceding comparison,
- if the preparative route is selected in the switching step, a preparative step according to the preparative route comprising:
- an operation of first pumping of a supercritical fluid or liquid and second pumping of a modifier,
- an operation of injection of at least one sample of the product P into at least one chromatography column, product P being eluted in the chromatography column by the supercritical fluid or liquid, and the modifier,
- an operation of separation of the constituents of the product P,
- an operation of detection of the constituents of the product P,
- an operation of collection of one or more constituents of the product P.
11 more elements
Dependencies are inferred from the claim text.