Synthesis of glucan comprising beta-1,3 glycosidic linkages with phosphorylase enzymes
AssigneeDanisco US Inc.
InventorsZHEYONG YU, SLAVKO KRALJ, NATNAEL BEHABTU, ZHENGHONG ZHANG, LAURIE A HOWE
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Abstract
Reaction compositions are disclosed herein comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with beta-1,3 glycosidic linkages. Further disclosed are methods of isolating beta-1,3-glucan.
Claims18
A reaction composition comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, or SEQ ID NO:12, wherein the enzyme synthesizes beta-1,3-glucan.
A method for producing beta-1,3-glucan, said method comprising:
- (a) contacting at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, or SEQ ID NO:12, wherein beta-1,3-glucan is produced;
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- (b) optionally, isolating the beta-1,3-glucan produced in step (a).
A method of processing beta-1,3-glucan, said method comprising:
- (a) providing an aqueous composition comprising up to about 40 wt % insoluble beta-1,3-glucan having a degree of polymerization (DP) of at least 17;
- (b) heating the aqueous composition to at least about 75° C., thereby dissolving the beta-1,3-glucan in the aqueous composition to provide a solution;
- (c) subjecting the solution to at least one process that reduces the content of one or more solutes other than the dissolved beta-1,3-glucan, thereby increasing the content of the dissolved beta-1,3-glucan in the solution on a dry weight basis;
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- (d) optionally cooling the solution to a temperature at which the beta-1,3-glucan precipitates back to an insoluble state, and optionally isolating the precipitated beta-1,3-glucan.
Dependencies are inferred from the claim text.