# US 2019/0317684 A1 · Dna digital data storage device and method, and decoding method of dna digital data > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0317684 A1 - Document ID: US20190317684A1 - Status: Published application - Kind: A1 - Application number: 16/138,123 - Filed: 2018-09-21 - Published: 2019-10-17 - Earliest claimed: 2018-04-11 - Assignees: University-Industry Cooperation Group of Kyung Hee University; Seoul National University R&Db Foundation - Applicants: University-Industry Cooperation Group of Kyung Hee University; Seoul National University R&DB Foundation - Inventors: Sunghoon Kwon; Wook Park; Yeongjae Choi; Taehoon Ryu; Suk-Heung Song; Hyeli Kim; Seojoo Kim - Web page: https://patentgrep.com/patent/US20190317684A1 ## CPC - G06F 3/0638: {Organizing or formatting or addressing of data} - C07H 21/02: with ribosyl as saccharide radical - C07H 21/04: with deoxyribosyl as saccharide radical - G06F 3/0604: {Improving or facilitating administration, e.g. storage management} - G06F 3/0673: {Single storage device} - H03M 7/30: Compression (speech analysis-synthesis for redundancy reduction G10L19/00; for image communication H04N); Expansion; Suppression of unnecessary data, e.g. redundancy reduction - G06F: ELECTRIC DIGITAL DATA PROCESSING (computer systems based on specific computational models G06N) - C07H: SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids C07C, C07D; aldonic acids, saccharic acids C07C59/105, C07C59/285; cyanohydrins C07C255/16; glycals C07D; compounds of unknown constitution C07G; polysaccharides, derivatives thereof C08B; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; sugar industry C13) - H03M: CODING; DECODING; CODE CONVERSION IN GENERAL (using fluidic means F15C4/00; optical analogue/digital converters G02F7/00; coding, decoding or code conversion, specially adapted for particular applications, see the relevant subclasses, e.g. G01D, G01R, G06F, G06T, G09G, G10L, G11B, G11C, H04B, H04L, H04M, H04N; ciphering or deciphering for cryptography or other purposes involving the need for secrecy G09C) ## Abstract Disclosed is a storage method of DNA digital data, including: encoding a plurality of bit data to a plurality of base sequences including at least one degenerate base; and synthesizing at least two types of bases constituting the at least one degenerate base on a substrate based on a mixing ratio. ## Claims ### Claim 1 A storage method of DNA digital data, the method comprising: encoding a plurality of bit data to a plurality of base sequences including at least one degenerate base; and synthesizing at least two types of bases constituting the at least one degenerate base on a substrate based on a mixing ratio. ### Claim 2 (depends on 1) The storage method of claim 1, further comprising: synthesizing a single type of base among the plurality of base sequences on the substrate. ### Claim 3 (depends on 1) The storage method of claim 1, wherein: the synthesizing of the at least one degenerate base includes, incorporating the at least two types of bases based on the mixing ratio onto the substrate. ### Claim 4 (depends on 1) The storage method of claim 1, wherein: the synthesizing of the at least one degenerate base includes, incorporating the at least two types of bases mixed according to the mixing ratio on the outside onto the substrate. ### Claim 5 (depends on 1) The storage method of claim 1, wherein: the ratios of the at least two types of bases are the same in the mixing ratio. ### Claim 6 (depends on 1) The storage method of claim 1, wherein: the ratios of the at least two types of bases are different in the mixing ratio. ### Claim 7 (depends on 1) The storage method of claim 1, wherein: the at least two types of bases are at least one of a DNA base, an RNA base, and a nucleic acid analogue. ### Claim 8 Device for storing data in DNA in which a plurality of base sequences to which a plurality of bit data is encoded is divided and stored as a basic storage unit, the device comprising: molecules in which a plurality of bases is synthesized based on a base sequence corresponding to the basic storage unit among the plurality of base sequences, wherein the base sequence includes a first degenerate base consisting of at least two types of bases, and the molecules include first molecules in which a base is synthesized among the at least two types of bases, and second molecules in which at least another base is synthesized among the at least two types of bases. ### Claim 9 (depends on 8) The storage device of claim 8, wherein: ratios between the first molecules and the second molecules follow a mixing ratio. ### Claim 10 (depends on 9) The storage device of claim 9, wherein: the ratios of the at least two types of bases are the same in the mixing ratio. ### Claim 11 (depends on 9) The storage device of claim 9, wherein: the ratios of the at least two types of bases are different in the mixing ratio. ### Claim 12 (depends on 8) The storage device of claim 8, wherein: the at least two types of bases are at least one of a DNA base, an RNA base, and a nucleic acid analogue. ### Claim 13 A decoding method of DNA data, comprising: categorizing a plurality of DNA fragments according to an address; analyzing a base at the same position with respect to each of the plurality of categorized DNA fragments; calculating a scatter plot of a base ratio based on a result of the analysis; determining a base at a corresponding location as a degenerate base when the scatter plot of the base ratio is a mixing ratio of at least two bases; and decoding data based on the determined degenerated base. ### Claim 14 (depends on 13) The decoding method of claim 13, wherein: the analyzing of the base includes, analyzing a base call for each location of each of the plurality of DNA fragments. ### Claim 15 (depends on 13) The decoding method of claim 13, wherein: the base at the corresponding location is determined as a first degenerate base when the scatter plot of the base ratio is a first mixing ratio of at least two bases, the base at the corresponding location is determined as a second degenerate base when the scatter plot of the base ratio is a second mixing ratio of the at least two bases, and the first mixing ratio and the second mixing ratio are different from each other and the first degenerate base and the second degenerate base are different from each other.