# US 2019/0317022 A1 · System and method of detecting contaminants

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- Number: US 2019/0317022 A1
- Document ID: US20190317022A1
- Status: Published application
- Kind: A1
- Application number: 16/280,802
- Filed: 2019-02-20
- Published: 2019-10-17
- Earliest claimed: 2018-02-20
- Assignees: Purdue Research Foundation
- Applicants: Purdue Research Foundation
- Inventors: Agbai NNANNA; Samuel OZEH; Justus NDUKAIFE
- Web page: https://patentgrep.com/patent/US20190317022A1

## CPC

- H04N 23/60: Control of cameras or camera modules
- G01N 21/0303: {Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment (G01N21/031 - G01N21/15 take precedence)}
- G01N 21/17: Systems in which incident light is modified in accordance with the properties of the material investigated (where the material investigated is optically excited causing a change in wavelength of the incident light G01N21/63)
- G01N 21/77: by observing the effect on a chemical indicator
- G06T 7/0002: {Inspection of images, e.g. flaw detection}
- G06T 7/90: Determination of colour characteristics
- G06V 10/25: Determination of region of interest [ROI] or a volume of interest [VOI]
- G01N 21/78: producing a change of colour
- G01N 31/22: using chemical indicators (G01N31/02 takes precedence)
- G01N 2021/0137: {with PC or the like}
- G01N 2021/0181: {Memory or computer-assisted visual determination}
- G01N 2021/0325: {Cells for testing reactions, e.g. containing reagents}
- G01N 2021/1765: {Method using an image detector and processing of image signal}
- G06F 17/18: for evaluating statistical data {, e.g. average values, frequency distributions, probability functions, regression analysis (forecasting specially adapted for a specific administrative, business or logistic context G06Q10/04)}
- G06V 10/56: relating to colour
- H04N: PICTORIAL COMMUNICATION, e.g. TELEVISION
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- G06T: IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06V: IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06F: ELECTRIC DIGITAL DATA PROCESSING (computer systems based on specific computational models G06N)

## Abstract

A system including a first container, wherein the first container includes a sample. The system includes a second container, wherein the second container comprises a reagent. Additionally, the system includes a test cell, wherein the test cell is configured to receive the sample and the reagent. Further, the reagent includes at least one of Griess reagent, sulfanilamide, or 1,5-diphenylcarbazide. Moreover, the test cell is situated in a chamber, wherein internal walls of the chamber are white.

## Claims

### Claim 1

A method comprising:
mixing a sample with a reagent, wherein the reagent comprises at least one of Griess reagent, sulfanilamide, or 1,5-diphenylcarbazide;
taking an image, using a camera, of the mixture of the sample with the reagent; and
analyzing the image.

### Claim 2 (depends on 1)

The method of claim 1, wherein the analyzing the image comprises analyzing the hue, saturation, and value (HSV) of the image.

### Claim 3 (depends on 1)

The method of claim 1, wherein the sample comprises at least one of nitrite, Chromium (VI), or hexavalent Chromium.

### Claim 4 (depends on 1)

The method of claim 1, wherein the camera is incorporated in a device, wherein the device is a cell-phone.

### Claim 5 (depends on 1)

The method of claim 1, wherein the camera is a 12 megapixel camera.

### Claim 6 (depends on 1)

The method of claim 1, wherein the camera comprises a f/2.2 aperture.

### Claim 7 (depends on 1)

The method of claim 1, wherein a ratio by volume of the sample to the reagent is 2, wherein the reagent is 1,5-diphenylcarbazide.

### Claim 8 (depends on 1)

The method of claim 1, wherein a ratio by volume of the sample to the reagent ranges from 0.5 to 1.5, wherein the reagent is the Griess reagent.

### Claim 9 (depends on 1)

The method of claim 1, wherein a ratio by volume of the sample to the reagent ranges from 1 to 2.5, wherein the reagent is 1,5-diphenylcarbazide.

### Claim 10 (depends on 1)

The method of claim 1, wherein a ratio by volume of the sample to the reagent is 1, wherein the reagent is Griess reagent.

### Claim 11

A method comprising:
mixing a sample with a reagent, wherein the reagent comprises at least one of Griess reagent, sulfanilamide, or 1,5-diphenylcarbazide, wherein the sample comprises at least one of nitrite, Chromium (VI), or hexavalent Chromium;
taking an image, using a camera, of the mixture of the sample with the reagent, wherein the camera is incorporated in a device, wherein the device is a cell-phone, wherein the camera is a 12 megapixel camera; and
analyzing the image, wherein the analyzing the image comprises analyzing the hue, saturation, and value (HSV) of the image.

### Claim 12 (depends on 11)

The method of claim 11, wherein the camera comprises a f/2.2 aperture.

### Claim 13 (depends on 11)

The method of claim 11, wherein a ratio by volume of the sample to the reagent is 2, wherein the reagent is 1,5-diphenylcarbazide.

### Claim 14 (depends on 11)

The method of claim 11, wherein a ratio by volume of the sample to the reagent ranges from 0.5 to 1.5, wherein the reagent is the Griess reagent.

### Claim 15 (depends on 11)

The method of claim 11, wherein a ratio by volume of the sample to the reagent ranges from 1 to 2.5, wherein the reagent is 1,5-diphenylcarbazide.

### Claim 16 (depends on 11)

The method of claim 11, wherein a ratio by volume of the sample to the reagent is 1, wherein the reagent is Griess reagent.

### Claim 17

A system comprising:
a first container, wherein the first container comprises a sample, wherein the sample comprises at least one of nitrite, Chromium (VI), or hexavalent Chromium;
a second container, wherein the second container comprises a reagent; and
a test cell, wherein the test cell is configured to receive the sample and the reagent, wherein the test cell is made of borosilicate glass,
wherein the reagent comprises at least one of Griess reagent, sulfanilamide, or 1,5-diphenylcarbazide,
wherein the test cell is situated in a chamber, wherein internal walls of the chamber are white, wherein the chamber comprises an LED strip, wherein the chamber is made of high density polyethylene, wherein the chamber comprises an opening.

### Claim 18 (depends on 17)

The system of claim 17, further comprising a camera, wherein the camera is configured to take an image of the test cell via the opening.

### Claim 19 (depends on 18)

The system of claim 18, wherein a distance between the camera and the test cell ranges from 100 millimeters (mm) to 120 mm.

### Claim 20 (depends on 17)

The system of claim 17, wherein a ratio by volume of the sample to the reagent is 2, wherein the reagent is 1,5-diphenylcarbazide.
