# US 2019/0316997 A1 · Extraction system for testing microbial contamination of tissue products > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0316997 A1 - Document ID: US20190316997A1 - Status: Published application - Kind: A1 - Application number: 16/381,531 - Filed: 2019-04-11 - Published: 2019-10-17 - Earliest claimed: 2018-04-11 - Assignees: AlloSource - Applicants: AlloSource - Inventors: Arthur Joslyn; Kenneth Blood; Jan Zajdowicz; Lauren Kapushion; Amy Kippen; Samantha White; Donald White - Web page: https://patentgrep.com/patent/US20190316997A1 ## CPC - B01L 3/508: {Rigid containers without fluid transport within} - G01N 1/4077: {by other techniques involving separation of suspended solids} - B01J 19/10: employing sonic or ultrasonic vibrations - C12Q 1/004: {mediator-assisted} - G01N 1/08: involving an extracting tool, e.g. core bit - B01L 3/502: {with fluid transport, e.g. in multi-compartment structures} - B01L 2300/042: Caps; Plugs - B01L 2300/0609: Holders integrated in container to position an object - B01L 2300/0681: Filter - B01L 2300/0832: cylindrical, tube shaped - G01N 2001/4094: {using ultrasound} - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES ## Abstract An extraction system for testing microbial contamination includes a biocompatible outer vessel that has a side wall and a biocompatible suspension system that is positionable within an interior of the biocompatible outer vessel. The biocompatible suspension system includes a horizontal member on which a sample may be supported and a securement mechanism that is engagable with the side wall of the biocompatible outer vessel to maintain the suspension system at a desired position within the biocompatible outer vessel. ## Claims ### Claim 1 An extraction system for testing microbial contamination, the system comprising: a biocompatible outer vessel comprising a side wall; and a biocompatible suspension system that is positionable within an interior of the biocompatible outer vessel, the biocompatible suspension system comprising: a horizontal member on which a tissue sample may be supported; and a securement mechanism that is engagable with the side wall of the biocompatible outer vessel to maintain the suspension system at a desired position within the biocompatible outer vessel. ### Claim 2 (depends on 1) The extraction system of claim 1, wherein: the securement mechanism comprises at least one hook that is configured to engage a top end of the side wall. ### Claim 3 (depends on 1) The extraction system of claim 1, wherein: the biocompatible suspension system comprises at least one vertical member that is coupled with the horizontal member and the securement mechanism. ### Claim 4 (depends on 1) The extraction system of claim 1, wherein: the biocompatible suspension system comprises a clamp that is coupled with the horizontal member, the clamp being configured to secure the tissue sample to the biocompatible suspension system. ### Claim 5 (depends on 4) The extraction system of claim 4, wherein: the clamp is moveable along a length of the horizontal member. ### Claim 6 (depends on 1) The extraction system of claim 1, wherein: the biocompatible outer vessel has a thickness of between about 0.5 and 3 millimeters. ### Claim 7 (depends on 1) The extraction system of claim 1, wherein: the biocompatible outer vessel comprises at least one of a spout, a flange, or a handle. ### Claim 8 An extraction system for testing microbial contamination, the system comprising: a biocompatible outer vessel; and one or both of: a biocompatible inner vessel positionable within the biocompatible outer vessel, the biocompatible inner vessel having a height of approximately thirteen inches and a diameter of approximately four inches, wherein the biocompatible inner vessel comprises a handle coupled to the biocompatible inner vessel; or a biocompatible suspension system positionable within the biocompatible outer vessel, wherein soft tissue is positionable on the biocompatible suspension system and wherein the extraction system is configured to receive a tissue sample having a size of at least thirty centimeters. ### Claim 9 (depends on 8) The extraction system of claim 8, wherein: the biocompatible suspension system comprises a curved portion that is configured to secure the biocompatible suspension system to a top end of the biocompatible outer vessel. ### Claim 10 (depends on 8) The extraction system of claim 8, wherein: the biocompatible outer vessel has a height of between about four inches and thirty inches. ### Claim 11 (depends on 8) The extraction system of claim 8, wherein: the biocompatible outer vessel comprises at least one of a spout, a flange, or a handle. ### Claim 12 (depends on 8) The extraction system of claim 8, wherein: the biocompatible outer vessel has a diameter of between approximately 2 inches and 8 inches. ### Claim 13 (depends on 8) The extraction system of claim 8, wherein: the biocompatible suspension system comprises: a first vertical member; a second vertical member; and a horizontal member that extends between and couples with the first vertical member and the second vertical member, the horizontal member being configured to support the tissue sample. ### Claim 14 (depends on 8) The extraction system of claim 8, wherein: the biocompatible inner vessel comprises a perforated sheet. ### Claim 15 A method of using an extraction system, comprising: securing a tissue sample to a horizontal member of a biocompatible suspension system; submerging at least a portion of the tissue sample in an extraction fluid provided within an interior of a biocompatible outer vessel; agitating the extraction fluid for a predetermined period of time to release microorganism from the tissue sample; removing the extraction fluid from the biocompatible outer vessel; and analyzing the extraction fluid for the presence of microbes. ### Claim 16 (depends on 15) The method of using an extraction system of claim 15, further comprising: removing the biocompatible suspension member and the tissue sample from the biocompatible outer vessel prior to removing the extraction fluid. ### Claim 17 (depends on 15) The method of using an extraction system of claim 15, wherein: the extraction fluid is agitated using a sonicator. ### Claim 18 (depends on 15) The method of using an extraction system of claim 15, wherein: securing the tissue sample to the horizontal member comprises clamping the tissue sample to the horizontal member using at least one clamp. ### Claim 19 (depends on 15) The method of using an extraction system of claim 15, wherein: the extraction fluid is removed from the biocompatible outer vessel while the extraction fluid is being agitated. ### Claim 20 (depends on 15) The method of using an extraction system of claim 15, wherein: submerging the at least a portion of the tissue sample in the extraction fluid comprises coupling a securement mechanism of the biocompatible suspension system to a top end of the biocompatible outer vessel.