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US 2019/0316174 A1Published application
Reaction accelerating agent
AssigneeKikkoman Corporation
InventorsAtsushi Ichiyanagi, Kazuhiko SHIMOJI
Life of this patent
Estimating the term…
Abstract
Provided is a compound which accelerates the enzymatic reaction catalyzed by an oxidase. The present invention provides an oxidase reaction accelerating agent comprising a compound represented by formula (I) and a method using the same.
Claims25
A method using oxidase reaction, comprising the step of using an oxidase and an oxidase reaction accelerating agent represented by the following formula (I):
- [Formula 1]
- R1—N═N—R2 (I)
- wherein
- R1 and R2 are each independently an aromatic 5- or 6-membered monocyclic carbocyclic ring or heterocyclic ring containing at least one nitrogen atom, sulfur atom or oxygen atom, or a 9- or 10-membered fused ring containing the aromatic 5- or 6-membered monocyclic carbocyclic ring or heterocyclic ring, wherein the ring may optionally be substituted with one or more substituents, wherein
- said substituent(s) is selected from the group consisting of —F, —Cl, —Br, —I, —At, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, ═O, —OH, —O—C1-6 alkyl, —CONH2, —NO2, —NH2, —CO—NH2, —R3, —NH—R4, —NHCO—NH—R5—N═N—R6, —SO3X, —COOX, Y, and Z;
- X is selected from the group consisting of —H, —Na, —K, and —Li;
- Y is selected from the group consisting of —H, —SO3X, and —COOX;
- Z is selected from the group consisting of —H, —C1-6 alkyl, —O—C1-6 alkyl, and —SO3X;
- each X, Y, and Z may be the same or different;
- —R3 is —H, or is an aromatic 5- or 6-membered monocyclic carbocyclic ring or heterocyclic ring containing at least one nitrogen atom, sulfur atom or oxygen atom, or a 9- or 10-membered fused ring containing the aromatic 5- or 6-membered monocyclic carbocyclic ring or heterocyclic ring, wherein the ring may optionally be substituted with one or more substituents selected from the group consisting of —F, —Cl, —Br, —I, —At, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, ═O, —OH, —O—C1-6 alkyl, —CONH2, —NO2, —NH2, and —SO3X;
- —R4 is an aromatic 5- or 6-membered monocyclic carbocyclic ring or heterocyclic ring containing at least one nitrogen atom, sulfur atom or oxygen atom, or a 9- or 10-membered fused ring containing the aromatic 5- or 6-membered monocyclic carbocyclic ring or heterocyclic ring, wherein the ring may optionally be substituted with one or more substituents selected from the group consisting of —F, —Cl, —Br, —I, —At, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, ═O, —OH, —O—C1-6 alkyl, —CONH2, —NO2, —NH2, —SO3X, —COOX, Y, and Z;
- and
- —R5 and —R6 are each independently an aromatic 5- or 6-membered monocyclic carbocyclic ring or heterocyclic ring containing at least one nitrogen atom, sulfur atom or oxygen atom, or a 9- or 10-membered fused ring containing the aromatic 5- to 6-membered monocyclic carbocyclic ring or heterocyclic ring, wherein the ring may optionally be substituted with one or more substituents selected from the group consisting of —F, —Cl, —Br, —I, —At, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, ═O, —OH, —O—C1-6 alkyl, —O—CH3, —CONH2, —NO2, —NH2, —SO3X, —COOX, Y, and Z,
- provided that when R1 or R2 is an aromatic 5-membered monocyclic carbocyclic ring or heterocyclic ring containing at least one nitrogen atom, sulfur atom or oxygen atom, the heterocyclic ring is not substituted with —COOX.
11 more elements
(canceled)
(canceled)
An oxidase reaction accelerating agent comprising a compound represented by formula (I):
- [Formula 20]
- R1—N═N—R2 (I)
- wherein
- R1 and R2 are each independently an aromatic 5- or 6-membered monocyclic carbocyclic ring or heterocyclic ring containing at least one nitrogen atom, sulfur atom or oxygen atom, or a 9- or 10-membered fused ring containing the aromatic 5- or 6-membered monocyclic carbocyclic ring or heterocyclic ring, wherein the ring may optionally be substituted with one or more substituents, wherein
- said substituent(s) is selected from the group consisting of —F, —Cl, —Br, —I, —At, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, ═O, —OH, —O—C1-6 alkyl, —CONH2, —NO2, —NH2, —CO—NH2, —R3, —NH—R4, —NHCO—NH—R5—N═N—R6, —SO3X, —COOX, Y, and Z;
- X is selected from the group consisting of —H, —Na, —K, and —Li;
- Y is selected from the group consisting of —H, —SO3X, and —COOX;
- Z is selected from the group consisting of —H, —C1-6 alkyl, —O—C1-6 alkyl, and —SO3X;
- each X, Y, and Z may be the same or different;
- —R3 is —H, or is an aromatic 5- or 6-membered monocyclic carbocyclic ring or heterocyclic ring containing at least one nitrogen atom, sulfur atom or oxygen atom, or a 9- or 10-membered fused ring containing the aromatic 5- or 6-membered monocyclic carbocyclic ring or heterocyclic ring, wherein the ring may optionally be substituted with one or more substituents selected from the group consisting of —F, —Cl, —Br, —I, —At, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, ═O, —OH, —O—C1-6 alkyl, —CONH2, —NO2, —NH2, and —SO3X;
- —R4 is an aromatic 5- or 6-membered monocyclic carbocyclic ring or heterocyclic ring containing at least one nitrogen atom, sulfur atom or oxygen atom, or a 9- or 10-membered fused ring containing the aromatic 5- or 6-membered monocyclic carbocyclic ring or heterocyclic ring, wherein the ring may optionally be substituted with one or more substituents selected from the group consisting of —F, —Cl, —Br, —I, —At, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, ═O, —OH, —O—C1-6 alkyl, —CONH2, —NO2, —NH2, —SO3X, —COOX, Y, and Z;
- and
- —R5 and —R6 are each independently an aromatic 5- or 6-membered monocyclic carbocyclic ring or heterocyclic ring containing at least one nitrogen atom, sulfur atom or oxygen atom, or a 9- or 10-membered fused ring containing the aromatic 5- to 6-membered monocyclic carbocyclic ring or heterocyclic ring, wherein the ring may optionally be substituted with one or more substituents selected from the group consisting of —F, —Cl, —Br, —I, —At, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, ═O, —OH, —O—C1-6 alkyl, —O—CH3, —CONH2, —NO2, —NH2, —SO3X, —COOX, Y, and Z,
- provided that when R1 or R2 is an aromatic 5-membered monocyclic carbocyclic ring or heterocyclic ring containing at least one nitrogen atom, sulfur atom or oxygen atom, the heterocyclic ring is not substituted with —COOX.
11 more elements
(canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.