Engineered viral vector reduces induction of inflammatory and immune responses
AssigneePresident and Fellows of Harvard College
InventorsYing Kai Chan, George Church
Life of this patent
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Abstract
Modified viral genomes are able to reduce induction of inflammatory and immune anti-viral responses. This manifests itself in reduced NF-kB activity, increased viral transduction rates, and increased expression of transgenes. Viral genomes are modified by incorporating one or more oligonucleotide sequences which are able to bind to TLR9 but not induce activation of it. The oligonucleotide sequences may be synthetic, bacterial, human, or from any other source.
Claims25
(canceled)
A recombinant viral genome comprising four repeated monomers of TTAGGG (SEQ ID NO:
- 6).
9-14. (canceled)
21-42. (canceled)
46-73. (canceled)
A method of producing a recombinant viral genome, comprising:
- inserting into a viral genome four repeated monomers of TTAGGG (SEQ ID NO:
- 6) or the nucleotide sequence of SEQ ID NO:
- 9.
A nucleic acid vector comprising the nucleotide sequence of SEQ ID NO:
- 9.
76-90. (canceled)
A recombinant viral genome comprising a sequence that is at least 95% identical to the sequence of SEQ ID NO:
- 9.
Dependencies are inferred from the claim text.