# US 2019/0316147 A1 · Pesticidal Genes and Methods of Use > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0316147 A1 - Document ID: US20190316147A1 - Status: Published application - Kind: A1 - Application number: 16/421,597 - Filed: 2019-05-24 - Published: 2019-10-17 - Earliest claimed: 2015-04-17 - Assignees: AgBiome, Inc. - Applicants: AgBiome, Inc. - Inventors: Jessica Parks; Kira Bulazel Roberts; Rebecca E. Thayer - Web page: https://patentgrep.com/patent/US20190316147A1 ## CPC - C12N 15/8286: {for insect resistance} - A01N 63/50: Isolated enzymes; Isolated proteins (peptides A01N37/46) - C07K 14/195: from bacteria - C07K 14/32: from Bacillus (G) - C07K 14/325: Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins - C12N 9/16: acting on ester bonds (3.1) - C12N 15/8285: {for nematode resistance} - C12Y 301/04011: Phosphoinositide phospholipase C (3.1.4.11) - Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - C12Y: ENZYMES - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, DNA constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides. Nucleotide sequences encoding the polypeptides can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided are useful for producing organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included. ## Claims ### Claim 1 A recombinant polypeptide, comprising (a) a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NOs: 5, 10, 24, 27, 40, 41, 45, 47, 49, 51, 52, 56, 59, 62, 64, 67, 77, 79, 80, 87, 92, 100, 102, 108, 111, 124, 129, 131, 132, 134, 136, 140, 148, 151, 156, 157, 159, 162, 164, 167, 172, 180, 181, 185, 199, 204, 208, 3, 7, 25, 28, 41, 49, 62, 79, 126, 132, 140, 172, 177, 180, 185, 191, or 199; or, (b) a polypeptide comprising the amino acid sequence set forth in SEQ ID NOs: 5, 10, 24, 27, 40, 41, 45, 47, 49, 51, 52, 56, 59, 62, 64, 67, 77, 79, 80, 87, 92, 100, 102, 108, 111, 124, 129, 131, 132, 134, 136, 140, 148, 151, 156, 157, 159, 162, 164, 167, 172, 180, 181, 185, 199, 204, 208, 3, 7, 25, 28, 41, 49, 62, 79, 126, 132, 140, 172, 177, 180, 185, 191, or 199. ### Claim 2 (depends on 1) The polypeptide of claim 1, further comprising a heterologous amino acid sequence. ### Claim 3 (depends on 1) A composition comprising the polypeptide of claim 1. ### Claim 4 A recombinant nucleic acid molecule encoding an amino acid sequence comprising (a) at least 90% sequence identity to an amino acid sequence set forth in SEQ ID Nos: 5, 10, 24, 27, 40, 41, 45, 47, 49, 51, 52, 56, 59, 62, 64, 67, 77, 79, 80, 87, 92, 100, 102, 108, 111, 124, 129, 131, 132, 134, 136, 140, 148, 151, 156, 157, 159, 162, 164, 167, 172, 180, 181, 185, 199, 204, 208, 3, 7, 25, 28, 41, 49, 62, 79, 126, 132, 140, 172, 177, 180, 185, 191, or 199; or (b) the amino acid sequence set forth in SEQ ID NOs: 5, 10, 24, 27, 40, 41, 45, 47, 49, 51, 52, 56, 59, 62, 64, 67, 77, 79, 80, 87, 92, 100, 102, 108, 111, 124, 129, 131, 132, 134, 136, 140, 148, 151, 156, 157, 159, 162, 164, 167, 172, 180, 181, 185, 199, 204, 208, 3, 7, 25, 28, 41, 49, 62, 79, 126, 132, 140, 172, 177, 180, 185, 191, or 199; wherein said recombinant nucleic acid molecule is not a naturally occurring sequence encoding said polypeptide. ### Claim 5 (depends on 4) The recombinant nucleic acid of claim 4, wherein said nucleic acid molecule is a synthetic sequence designed for expression in a plant. ### Claim 6 (depends on 4) The recombinant nucleic acid molecule of claim 4, wherein said nucleic acid molecule is operably linked to a promoter capable of directing expression in a plant cell. ### Claim 7 (depends on 4) The recombinant nucleic acid molecule of claim 4, wherein said nucleic acid molecule is operably linked to a promoter capable of directing expression in a bacteria. ### Claim 8 (depends on 4) A host cell comprising the recombinant nucleic acid molecule of claim 4. ### Claim 9 (depends on 8) The host cell of claim 8, wherein said host cell is a bacterial host cell. ### Claim 10 A DNA construct comprising a promoter that drives expression in a plant cell operably linked to a recombinant nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NOs: 5, 10, 24, 27, 40, 41, 45, 47, 49, 51, 52, 56, 59, 62, 64, 67, 77, 79, 80, 87, 92, 100, 102, 108, 111, 124, 129, 131, 132, 134, 136, 140, 148, 151, 156, 157, 159, 162, 164, 167, 172, 180, 181, 185, 199, 204, 208, 3, 7, 25, 28, 41, 49, 62, 79, 126, 132, 140, 172, 177, 180, 185, 191, or 199. ### Claim 11 (depends on 10) The DNA construct of claim 10, wherein said nucleotide sequence is a synthetic DNA sequence designed for expression in a plant. ### Claim 12 (depends on 10) A vector comprising the DNA construct of claim 10. ### Claim 13 (depends on 12) A host cell comprising the vector of claim 12. ### Claim 14 (depends on 13) A composition comprising the host cell of claim 13. ### Claim 15 (depends on 14) The composition of claim 14, wherein said composition is selected from the group consisting of a powder, dust, pellet, granule, spray, emulsion, colloid, and solution. ### Claim 16 (depends on 15) The composition of claim 15, wherein said composition comprises from about 1% to about 99% by weight of said polypeptide. ### Claim 17 (depends on 3) A method for controlling a pest population comprising contacting said pest population with a pesticidal-effective amount of the composition of claim 3. ### Claim 18 (depends on 13) A method for producing a polypeptide comprising culturing the host cell of claim 13 under conditions in which the nucleic acid molecule encoding the polypeptide is expressed. ### Claim 19 A plant having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein, wherein said nucleotide sequence comprises (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NOs: 5, 10, 24, 27, 40, 41, 45, 47, 49, 51, 52, 56, 59, 62, 64, 67, 77, 79, 80, 87, 92, 100, 102, 108, 111, 124, 129, 131, 132, 134, 136, 140, 148, 151, 156, 157, 159, 162, 164, 167, 172, 180, 181, 185, 199, 204, 208, 3, 7, 25, 28, 41, 49, 62, 79, 126, 132, 140, 172, 177, 180, 185, 191, or 199; or (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NOs: 5, 10, 24, 27, 40, 41, 45, 47, 49, 51, 52, 56, 59, 62, 64, 67, 77, 79, 80, 87, 92, 100, 102, 108, 111, 124, 129, 131, 132, 134, 136, 140, 148, 151, 156, 157, 159, 162, 164, 167, 172, 180, 181, 185, 199, 204, 208, 3, 7, 25, 28, 41, 49, 62, 79, 126, 132, 140, 172, 177, 180, 185, 191, or 199. ### Claim 20 (depends on 19) A transgenic seed of the plant of claim 19. ### Claim 21 A method for protecting a plant from an insect pest, comprising expressing in a plant or cell thereof a nucleotide sequence that comprises (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NOs: 5, 10, 24, 27, 40, 41, 45, 47, 49, 51, 52, 56, 59, 62, 64, 67, 77, 79, 80, 87, 92, 100, 102, 108, 111, 124, 129, 131, 132, 134, 136, 140, 148, 151, 156, 157, 159, 162, 164, 167, 172, 180, 181, 185, 199, 204, 208, 3, 7, 25, 28, 41, 49, 62, 79, 126, 132, 140, 172, 177, 180, 185, 191, or 199; or (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NOs: 5, 10, 24, 27, 40, 41, 45, 47, 49, 51, 52, 56, 59, 62, 64, 67, 77, 79, 80, 87, 92, 100, 102, 108, 111, 124, 129, 131, 132, 134, 136, 140, 148, 151, 156, 157, 159, 162, 164, 167, 172, 180, 181, 185, 199, 204, 208, 3, 7, 25, 28, 41, 49, 62, 79, 126, 132, 140, 172, 177, 180, 185, 191, or 199. ### Claim 22 (depends on 21) The method of claim 21, wherein said plant produces a pesticidal polypeptide having pesticidal activity against at least one of a lepidopteran pest, a coleopteran pest, or a hemipteran pest. ### Claim 23 A method for increasing yield in a plant comprising growing in a field a plant or seed thereof having stably incorporated into its genome a DNA construct comprising a promoter that drives expression in a plant operably linked to a nucleotide sequence, wherein said nucleotide sequence comprises (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NOs: 5, 10, 24, 27, 40, 41, 45, 47, 49, 51, 52, 56, 59, 62, 64, 67, 77, 79, 80, 87, 92, 100, 102, 108, 111, 124, 129, 131, 132, 134, 136, 140, 148, 151, 156, 157, 159, 162, 164, 167, 172, 180, 181, 185, 199, 204, 208, 3, 7, 25, 28, 41, 49, 62, 79, 126, 132, 140, 172, 177, 180, 185, 191, or 199; or (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NOs: 5, 10, 24, 27, 40, 41, 45, 47, 49, 51, 52, 56, 59, 62, 64, 67, 77, 79, 80, 87, 92, 100, 102, 108, 111, 124, 129, 131, 132, 134, 136, 140, 148, 151, 156, 157, 159, 162, 164, 167, 172, 180, 181, 185, 199, 204, 208, 3, 7, 25, 28, 41, 49, 62, 79, 126, 132, 140, 172, 177, 180, 185, 191, or 199.