# US 2019/0316080 A1 · Date Palm Medium Compositions and Methods

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- Number: US 2019/0316080 A1
- Document ID: US20190316080A1
- Status: Published application
- Kind: A1
- Application number: 16/387,274
- Filed: 2019-04-17
- Published: 2019-10-17
- Earliest claimed: 2018-04-17
- Assignees: Bien-Etre Labs, LLC
- Applicants: Bien-Etre Labs, LLC
- Inventors: Adnan Mjalli; Assel T. Issa; Salam A. Ibrahim; Lorraine M. Rusch; Ahmad Abuzuaiter
- Web page: https://patentgrep.com/patent/US20190316080A1

## CPC

- B01D 11/0288: {Applications, solvents}
- C12N 1/20: Bacteria; Culture media therefor
- A23L 33/135: Bacteria or derivatives thereof, e.g. probiotics
- A23L 19/09: {Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks (A23L19/01, A23L19/10, A23L27/63 and A23L21/00 take precedence)}
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A23V 2400/169: Plantarum
- A23V 2400/173: Reuteri
- A23V 2400/175: Rhamnosus
- A23V 2400/515: Animalis
- A23V 2400/517: Bifidum
- A23V 2400/533: Longum
- B01D 11/0253: {Fluidised bed of solid materials}
- C12N 2500/76: from plants
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

Disclosed are date palm compositions and methods of use of such compositions. Such compositions may be used as part of a growth medium for culture of microorganisms. In certain embodiments, date palm extracts of the disclosure are used as part a culture medium to grow Lactobacilli.

## Claims

### Claim 1

A method of preparing a date palm extract (DPE) broth comprising:
(i) obtaining a mass of date material;
(ii) combining the date material with a suspension liquid to produce a slurry mixture;
(iii) processing the slurry mixture to produce a date palm extract broth with fibers (DPEF); and
(iv) separating the DPEF into the DPE broth and a mass of date palm fiber (DPF).

### Claim 2 (depends on 1)

The method of claim 1, wherein the date material comprises a date paste.

### Claim 3 (depends on 1)

The method of claim 1, wherein the processing step comprises at least one heating period, at least one milling period, and at least one soaking period.

### Claim 4 (depends on 3)

The method of claim 3, wherein the heating period comprises mixing the slurry mixture while heating the slurry mixture to a temperature of at least 60° C. for at least 3 hours.

### Claim 5 (depends on 4)

The method of claim 4, wherein the slurry mixture is mixed under high shear agitation.

### Claim 6 (depends on 5)

The method of claim 5, wherein the milling period comprises milling the heated slurry mixture using a colloid mill.

### Claim 7 (depends on 6)

The method of claim 6, wherein the soaking period comprises diluting the milled slurry mixture with the suspension liquid and cooling the diluted, milled slurry mixture at 4° C. for at least 12 hours.

### Claim 8 (depends on 1)

The method of claim 1, wherein the processing step comprises:
(i) a heating period comprising mixing the slurry mixture under high shear agitation while heating the slurry mixture to a temperature between 65° C. and 80° C. for between 45 and 90 minutes;
(ii) a milling period comprising milling the heated slurry mixture three times using a colloid mill; and
(iii) a soaking period comprising diluting the milled slurry mixture with the suspension liquid and cooling the diluted, milled slurry mixture at 4° C. for at least 12 hours.

### Claim 9

A method of preparing a date palm medium (DPM) for culturing microorganisms comprising:
(i) preparing a buffer solution;
(ii) buffering a date palm extract (DPE) broth with the buffer solution; and
(iii) adding a nitrogen source from non-animal origin to the buffered DPE broth.

### Claim 10

A method of preparing a date palm medium with fiber (DPFM) for culturing microorganisms comprising:
(i) preparing a buffer solution;
(ii) buffering a date palm extract broth with fibers (DPEF) with the buffer solution; and
(iii) adding a nitrogen source from non-animal origin to the buffered DPEF.

### Claim 11 (depends on 10)

The method of claim 10, wherein the buffer solution is prepared by combining L-cysteine hydrochloride, sodium phosphate, ammonium citrate, sodium acetate, calcium chloride, potassium phosphate, magnesium sulfate heptahydrate, manganese sulfate tetrahydrate, arginine, TWEEN-80®, and deionized water.

### Claim 12 (depends on 11)

The buffer solution of claim 11, wherein the concentration of calcium chloride is at least 0.2 g/L.

### Claim 13 (depends on 11)

The buffer solution of claim 11, wherein the concentration of TWEEN-80® is at least 2 mL/L.

### Claim 14 (depends on 10)

The method of claim 10, wherein the buffering step comprises combining the buffer solution and the DPE or DPEF solution in a volume to volume ratio of 2:3.

### Claim 15 (depends on 10)

The method of claim 10, wherein the nitrogen source comprises a peptone, tryptone, proteose peptone, phytone peptone, polypeptone peptonetryptic soy broth, or a yeast extract.

### Claim 16 (depends on 10)

The method of claim 10, wherein an amount of fiber is added to the DPM or DPFM.

### Claim 17

A method of cultivating microorganisms comprising:
(i) inoculating a date palm medium (DPM) with microorganisms; and
(ii) incubating the microorganisms under conditions such that growth occurs.

### Claim 18

A method of cultivating microorganisms comprising:
(i) inoculating a date palm medium plus fiber (DPFM) with microorganisms; and
(ii) incubating the microorganisms under conditions such that growth occurs.

### Claim 19 (depends on 18)

The method of claim 18, wherein the microorganisms are bacteria.

### Claim 20 (depends on 19)

The method of claim 19, wherein the bacteria is at least one of a Lactobacillus species or a Bifidobacterium species.

### Claim 21 (depends on 18)

The method of claim 18, wherein the DPFM is inoculated with 1-2% microorganisms by volume.

### Claim 22

A probiotic comprising bacteria cultivated by the steps of: (i) inoculating a date palm medium plus fiber (DPFM) with microorganisms; and (ii) incubating the microorganisms under conditions such that growth occurs.

### Claim 23 (depends on 22)

The probiotic of claim 22, wherein the bacteria is at least one of a Lactobacillus species or a Bifidobacterium species.

### Claim 24 (depends on 22)

The probiotic of claim 22 wherein the probiotic is a dietary supplement or a food supplement.