# US 2019/0316079 A1 · Pairing probiotics and prebiotics, methods for growth and use, separately and in combination > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0316079 A1 - Document ID: US20190316079A1 - Status: Published application - Kind: A1 - Application number: 16/380,226 - Filed: 2019-04-10 - Published: 2019-10-17 - Earliest claimed: 2018-04-11 - Inventors: Shayne Morris; Michael Benson - Web page: https://patentgrep.com/patent/US20190316079A1 ## CPC - C12N 1/20: Bacteria; Culture media therefor - C12N 1/04: Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract A method for growing probiotic organisms wherein the growth media includes prebiotics especially selected and prepared to be paired with the probiotic organisms being grown. The prebiotic formula is optimized to grow the desired probiotic organisms, as well as important byproducts of the growth process. Specialized freeze-drying buffers may also be paired with certain probiotic organisms for the freeze-drying process. ## Claims ### Claim 1 A method for growing probiotic organisms comprising: selecting at least one probiotic organism to grow; selecting a growth broth for growing the probiotic organism, wherein the growth broth includes a prebiotic formula that is optimized for growing the probiotic organism; inoculating the growth broth with the probiotic organism; growing the probiotic organism in the growth broth; harvesting the probiotic organism from the growth broth; selecting an agar spread plate for growing the probiotic organism, wherein the agar spread plate media includes a prebiotic formula that is optimized for growing the probiotic organism; inoculating the agar spread plate with the probiotic organism harvested from the growth broth; growing the probiotic organism on the agar spread plate; and harvesting the probiotic organism from the agar spread plate. ### Claim 2 (depends on 1) The method of claim 1, further comprising: freeze-drying the probiotic organism harvested from the agar spread plate by use of a lyophilization reagent, wherein the lyophilization reagent includes at least two compounds selected from the group consisting of glucosamine, glutamine, sucrose, mannitol, trehalose, glycerol, inositol, raffinose, inulin, powdered skim milk, activated charcoal, soluble starch, collagen powder, chondroitin sulfate, glucosamine sulfate, guar, acacia, silica, and fructooligosaccharide P95. ### Claim 3 (depends on 1) The method of claim 1, wherein the at least one probiotic organism to grow is selected from the group consisting of Faecalibacterium prausnitzii, Mycobacterium vaccae, and Lactobacillus farciminis, and the prebiotic formula included in the growth broth and in the agar spread plate media is Prebiotic Formula Alpha. ### Claim 4 (depends on 1) The method of claim 1, wherein the at least one probiotic organism to grow is selected from the group consisting of Eubacterium limosum, Bacteroides thetaiotaomicron, Lactobacillus vaginalis, and Bacteroides uniformis, and the prebiotic formula included in the growth broth and in the agar spread plate media is Prebiotic Formula Beta. ### Claim 5 (depends on 1) The method of claim 1, wherein the at least one probiotic organism to grow is selected from the group consisting of Bacteroides ovatus, Anaerostipes caccae, Staphylococcus xylosus, Staphylococcus epidermidis, and Roseomonas mucosa, and the prebiotic formula included in the growth broth and in the agar spread plate media is Prebiotic Formula Gamma. ### Claim 6 (depends on 1) The method of claim 1, wherein the at least one probiotic organism to grow is selected from the group consisting of Collinsella aerofaciens, Bacteroides ovatus, Bacteroides uniformis, Clostridium symbiosum, Bacteroides fragilis, and Roseburia hominis, and the prebiotic formula included in the growth broth and in the agar spread plate media is Prebiotic Formula Delta. ### Claim 7 (depends on 1) The method of claim 1, wherein at least two probiotic organisms are selected from the group consisting of Faecalibacterium prausnitzii, Mycobacterium vacae, and Lactobacillus farciminis, and the prebiotic formula included in the growth broth and in the agar spread plate media is Prebiotic Formula Alpha. ### Claim 8 (depends on 1) The method of claim 1, wherein at least two probiotic organisms are selected from the group consisting of Eubacterium limosum, Bacteroides thetaiotaomicron, Lactobacillus vaginalis, and Bacteroides uniformis, and the prebiotic formula included in the growth broth and in the agar spread plate media is Prebiotic Formula Beta. ### Claim 9 (depends on 1) The method of claim 1, further comprising: freeze-drying the probiotic organisms harvested from the agar spread plate by use of a Freeze-drying Buffer A when the probiotic organism is at least one probiotic organism selected from the group consisting of Mycobacterium vaccae, Pediococcus acidilactici, Roseomonas mucosa, Lactobacillus farciminis, Staphylococcus epidermidis, and Staphylococcus xylosus. ### Claim 10 (depends on 1) The method of claim 1, further comprising: freeze-drying the probiotic organisms harvested from the agar spread plate by use of a Freeze-drying Buffer B when the probiotic organism is at least one probiotic organism selected from the group consisting of Bacteroides thetaiotaomicron, Bacteroides fragilis, Bacteroides ovatus, Bacteroides uniformis, Eubacterium limosum, Collinsella aerofaciens, Akkermansia muciniphila, and Roseburia hominis. ### Claim 11 (depends on 1) The method of claim 1, further comprising: freeze-drying the probiotic organisms harvested from the agar spread plate by use of a Freeze-drying Buffer C when the probiotic organism is at least one probiotic organism selected from the group consisting of Clostridium symbiosum, Faecalibacterium praunsnitzii, Eubacterium rectale, Anaerostipes caccae, and Butyricicoccus pullicaecorum. ### Claim 12 (depends on 1) The method of claim 1, further comprising: freeze-drying the probiotic organism harvested from the agar spread plate by use of a Freeze-drying Buffer D when the probiotic organism is Desulfovibrio piger. ### Claim 13 A method for growing a probiotic organism comprising: selecting a probiotic organism to grow, wherein the probiotic organism is selected from the group consisting of Bacteroides ovatus, Anaerostipes caccae, Staphylococcus xylosus, Staphylococcus epidermidis, and Roseomonas mucosa; selecting a growth broth for growing the probiotic organism, wherein the growth broth is comprised of 1%-15% Prebiotic Formula Gamma; inoculating the growth broth with the probiotic organism; growing the probiotic organism in the growth broth; harvesting the probiotic organism from the growth broth; selecting an agar spread plate for growing the probiotic organism, wherein the agar spread plate media is comprised of 1%-15% Prebiotic Formula Gamma; inoculating the agar spread plate with the probiotic organism harvested from the growth broth; growing the probiotic organism on the agar spread plate; and harvesting the probiotic organism from the agar spread plate. ### Claim 14 (depends on 13) The method of claim 13 further comprising: freeze-drying the probiotic organism. ### Claim 15 (depends on 13) The method of claim 13, further comprising: freeze-drying the probiotic organism by use of a Freeze-drying Buffer A when the probiotic organism is Staphylococcus epidermidis or Staphylococcus xylosus. ### Claim 16 A method for growing a probiotic organism comprising: selecting a probiotic organism to grow, wherein the probiotic organism is selected from the group consisting of Collinsella aerofaciens, Bacteroides fragilis, Bacteroides ovatus, Bacteroides uniformis, Clostridium symbiosum, and Roseburia hominis; selecting a growth broth for growing the probiotic organism, wherein the growth broth is comprised of 1%-15% Prebiotic Formula Delta; inoculating the growth broth with the probiotic organism; growing the probiotic organism in the growth broth; harvesting the probiotic organism from the growth broth; selecting an agar spread plate for growing the probiotic organism, wherein the agar spread plate media is comprised of 1%-15% Prebiotic Formula Delta; inoculating the agar spread plate with the probiotic organism harvested from the growth broth; growing the probiotic organism on the agar spread plate; and harvesting the probiotic organism from the agar spread plate. ### Claim 17 (depends on 16) The method of claim 16, further comprising: freeze-drying the probiotic organism. ### Claim 18 (depends on 16) The method of claim 16, further comprising: freeze-drying the probiotic organism by use of a Freeze-drying Buffer B when the probiotic organism is a probiotic organism selected from the group consisting of Bacteroides fragilis, Bacteroides ovatus, Bacteroides uniformis, Collinsella aerofaciens, and Roseburia hominis. ### Claim 19 (depends on 16) The method of claim 16, further comprising: freeze-drying the probiotic organism by use of a Freeze-drying Buffer C when the probiotic organism is Clostridium symbiosum.