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US 2019/0315790 A1Published application
Synthesis of d-allulose
AssigneePfeifer & Langen
InventorsTimo Johannes Koch, Florian Kipping, Steffen Butz, Marcel Lesch, Thomas Hässler
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Abstract
The invention relates to a process for the synthesis of a product saccharide, preferably of D-allulose from an educt saccharide, preferably from D-fructose under heterogeneous or homogeneous catalysis which includes chemical and/or enzymatic catalysis. The synthesis is performed in at least two reactors that are arranged in series and the reaction product exiting the first reactor is subjected to chromatographic separation before it enters the second reactor. Preferably, the chromatographic separation is integrated in a simulated moving bed.
Claims32
1:
- A process for the synthesis of product saccharide in at least two reactors R1 and R2, the method comprising the steps of
- (i) supplying a liquid comprising educt saccharide to the reactor R1 and converting a portion of the educt saccharide to product saccharide under enzymatic catalysis thereby providing a liquid comprising product saccharide and residual educt saccharide;
- (ii) separating at least a portion of the product saccharide from the residual educt saccharide of step (i) by liquid chromatography thereby providing
- a first chromatographic fraction comprising residual educt saccharide and optionally product saccharide;
- and
- a second chromatographic fraction comprising product saccharide and optionally residual educt saccharide;
- and
- (iii) supplying the first chromatographic fraction of step (ii) to the reactor R2 and converting at least a portion of the residual educt saccharide to product saccharide under enzymatic catalysis.
5 more elements
2-4. (canceled)
5:
- The process according to claim 1, wherein
- (i) the educt saccharide is glucose, the product saccharide is fructose, and the conversions according to step (i) and/or step (iii) are performed under enzymatic catalysis by glucose-fructose-epimerase;
- or
- (ii) the educt saccharide is fructose, the product saccharide is tagatose, and the conversions according to step (i) and/or step (iii) are performed under enzymatic catalysis by tagatose-3-epimerase;
- or
- (iii) the educt saccharide is a monosaccharide, preferably galactose, the product saccharide is a monosaccharide, preferably tagatose, and the conversions according to step (i) and/or step (iii) are performed under enzymatic catalysis by tagatose-3-epimerase;
- or
- (iv) the educt saccharide is a mixture of glucose-1-phosphate and glucose, the product saccharide is cellobiose, and the conversions according to step (i) and/or step (iii) are performed under enzymatic catalysis by cellobiose phosphorylase;
- or
- (v) the educt saccharide is a mixture of glucose-1-phosphate and fructose, the product saccharide is sucrose, and the conversions according to step (i) and/or step (iii) are performed under enzymatic catalysis by sucrose phosphorylase.
7 more elements
6-9. (canceled)
10:
- The process according to claim 1, wherein the second chromatographic fraction is supplied to the reactor R2 after the first chromatographic fraction.
11-17. (canceled)
18:
- The process according to claim 1, wherein reactor R1 and/or reactor R2 is a membrane reactor or a chromatographic reactor, preferably an immobilized column reactor.
19:
- The process according to claim 1, which is performed continuously.
20:
- The process according to claim 1, wherein the liquid chromatography of step (ii) is integrated in a simulated moving bed (SMB).
21:
- The process according to claim 20, wherein liquid flows through the SMB in a flow direction and an adsorbent bed is simulated to move in opposite direction.
22:
- The process according to claim 20, wherein the SMB comprises four zones I to IV, wherein liquid is cycled through zones I to IV and wherein with respect to flow direction of liquid zone IV is downstream zone III, zone III is downstream zone II, zone II is downstream zone I, and zone I is downstream zone IV.
(canceled)
24:
- The process according to claim 20, wherein the SMB comprises
- a zone I comprising one or more serial adsorbent beds C-Im, wherein index m is an integer of at least 1;
- a zone II comprising one or more serial adsorbent beds C-IIn, wherein index n is an integer of at least 1;
- a zone III comprising the reactor R1 for the conversion of step (i), the reactor R2 for the conversion of step (iii), and one or more serial adsorbent beds C-IIIp, wherein index p is an integer of at least 1;
- wherein with respect to flow direction of liquid (eluent) at least one of said adsorbent beds C-IIIp is arranged downstream the reactor R1 and upstream the reactor R2;
- and
- a zone IV comprising one or more serial adsorbent beds C-IVq, wherein index p is an integer of at least 1.
4 more elements
25:
- The process according to claim 24, wherein indices m, n, p and q are independently of one another within the range of from 1 to 12.
26:
- The process according to claim 24, wherein at least one of indices m, n, p and q is greater than 1.
27:
- The process according to claim 24, wherein indices m, n, p and q are identical.
28:
- The process according to claim 24, wherein indices m, n, p and q are not identical.
29-31. (canceled)
32:
- The process according to claim 1, which comprises the additional step of filtering the liquid by means of a filter.
(canceled)
34:
- The process according to claim 1, which comprises the additional step of decoloring the liquid by means of a decolorizer.
(canceled)
36:
- The process according to claim 1, which comprises the additional step of regulating the pH of the liquid by means of a pH regulator.
(canceled)
38:
- The process according to claim 1, which comprises the additional step of concentrating the liquid by means of a concentrator.
(canceled)
40:
- The process according to claim 1, which comprises the additional step of desalting the liquid by means of a desalter.
(canceled)
42:
- An apparatus for performing the process according to claim 1, comprising the following components in liquid flow communication:
- (I) a reactor R1 which comprises an enzyme capable of converting educt saccharide to product saccharide;
- (II) a first chromatography unit after reactor R1 for separating product saccharide from educt saccharide;
- and
- (III) a reactor R2 after the first chromatography unit, wherein the reactor R2 also comprises an enzyme capable of converting educt saccharide to product saccharide.
2 more elements
43-46. (canceled)
47:
- The apparatus according to claim 42, which additionally comprises in liquid flow communication:
- means for recirculating educt saccharide to the reactor R1;
- and/or
- a filter;
- and/or
- a decolorizer;
- and/or
- a pH regulator;
- and/or
- a concentrator;
- and/or
- a desalter.
9 more elements
(canceled)
Dependencies are inferred from the claim text.