New compounds and uses thereof for detection of target molecules in a sample

A pro-fluorophore of Formula (I):

• wherein R1 to R8, R11-R12 and R14-R15 are independently selected from hydrogen, hydroxyl, halogen, cyano, nitro, optionally substituted C1-C10 alkyl, optionally substituted amino C1-C10 alkyl, optionally substituted C1-C10 alkoxy, R13 is selected from optionally substituted C1-C10 alkyl, optionally substituted ethyl, optionally substituted propyl or optionally substituted butyl, Z is —CR16R17 wherein R16 and R17 are independently selected from hydrogen and optionally substituted C1-C6 alkyl, or any tautomer, isomer, conjugate or salts thereof.

A fluorophore according to Formula (I)′ wherein said fluorophore is selected from the following group:

• methyl 2-(3-(6-chloro-4-oxo-3,4-dihydroquinazolin-2-yl)-4-hydroxyphenyl)acetate (10);
• and
• 2-(3-(6-chloro-4-oxo-3,4-dihydroquinazolin-2-yl)-4-hydroxy phenyl)acetic acid (11).