# US 2019/0314813 A1 · A point-of-care diagnostic assay cartridge > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0314813 A1 - Document ID: US20190314813A1 - Status: Published application - Kind: A1 - Application number: 16/343,176 - Filed: 2017-10-25 - Published: 2019-10-17 - Earliest claimed: 2016-10-26 - Assignees: Radisens Diagnostics Limited - Applicants: Radisens Diagnostics Limited - Inventors: David Doolan; Donal Cronin; Eoin O'Nuallain - Web page: https://patentgrep.com/patent/US20190314813A1 ## CPC - B01L 3/50273: {characterised by the means or forces applied to move the fluids} - B01L 2200/0621: Control of the sequence of chambers filled or emptied - B01L 2200/16: Reagents, handling or storing thereof - B01L 2300/0803: Disc shape - B01L 2400/0409: centrifugal forces - B01L 2400/0457: passive flow or gravitation - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ## Abstract The invention provides a microfluidic system comprising a cartridge coupled to a motor and adapted to move a fluid sample to a plurality of locations on the cartridge. The cartridge comprises a chevron shaped or substantially V shaped reaction chamber having at least three zones, a first zone positioned near the apex of the V shaped reaction chamber to define a detection zone, a second zone positioned near a first end of the V shaped reaction chamber and a third zone positioned near a second end of the V shaped reaction chamber. The motor and a control module is configured to provide a combination of centrifugal force and gravitational force to move said fluid sample between at least three zones. ## Claims ### Claim 1 A microfluidic system comprising: a cartridge coupled to a motor and adapted to move a fluid sample to a plurality of locations on the cartridge, wherein the cartridge is configured to rotate on an inclined plane with respect to a horizontal plane; the cartridge comprises a chevron shaped or substantially V shaped reaction chamber having at least three zones, a first zone positioned near the apex of the V shaped reaction chamber to define a detection zone, a second zone positioned near a first end of the V shaped reaction chamber and a third zone positioned near a second end of the V shaped reaction chamber, wherein each of the second zone and the third zone comprise a reagent zone; and the motor and a control module is configured to provide a combination of centrifugal force and gravitational force to move said fluid sample between the at least three zones. ### Claim 2 (depends on 1) The microfluidic system as claimed in claim 1 wherein the first zone comprises a cuvette and is positioned adjacent to the outer diameter of the cartridge. ### Claim 3 (depends on 1) The microfluidic system as claimed in claim 1 wherein the V shaped chamber extends radially inward from the first zone on two sides to create two zones that can be independently filled with fluid to define the second zone and third zone. ### Claim 4 (depends on 1) The microfluidic system as claimed in claim 1 wherein the cartridge is configured to rotate on the inclined plane at a velocity such that a combination of centrifugal force and gravity influence the movement of the fluid sample radially outward and inward respectively in operation. ### Claim 5 (depends on 1) The microfluidic system as claimed in claim 1 wherein the cartridge is configured to rotate at a velocity such that the relative centrifugal force (RCF) is greater than gravity, and the fluid sample can be moved radially outward on the cartridge. ### Claim 6 (depends on 1) The microfluidic system as claimed in claim 1 wherein the cartridge is configured such that no fluid reaches the second zone or third zone when the fluid sample is under the influence of the centrifugal force. ### Claim 7 (depends on 1) The microfluidic system as claimed in claim 1 wherein when the cartridge is stationary or configured to rotate slowly, gravity will influence the fluid and move the fluid towards the second zone or third zone. ### Claim 8 (depends on 7) The microfluidic system as claimed in claim 7 wherein the cartridge is further configurable to be agitated to overcome any effects of surface tension that may prevent the fluid from flowing under the influence of gravity. ### Claim 9 (depends on 1) The microfluidic system as claimed in claim 1 wherein the second zone comprises a dried reagent. ### Claim 10 (depends on 1) The microfluidic system as claimed in claim 1 wherein the third zone comprises a dried reagent. ### Claim 11 (depends on 9) The microfluidic system as claimed in claim 9 wherein the cartridge is configured such that the dried reagent remains intact until the second or third zones are rehydrated with the fluid sample and a buffer solution. ### Claim 12 (depends on 9) The microfluidic system as claimed in claim 9 wherein the dried reagent can be spotted in singular or multiple spots in said second and/or third zones. ### Claim 13 (depends on 9) The microfluidic system as claimed claim 9 wherein the second or third zone comprises multiple dried reagents. ### Claim 14 (depends on 11) The microfluidic system as claimed in claim 11, wherein the cuvette comprises a single volume cuvette configured to allow for optical measurement of the buffer solution, the fluid sample and the rehydrated reagents used in each phase of an assay. ### Claim 15 (depends on 1) The microfluidic system as claimed in claim 1, wherein the system is configured for performing an immunoturbidimetric or an enzyme-based clinical chemistry assay.