# US 2019/0314778 A1 · Mixing device for cell printing composition > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0314778 A1 - Document ID: US20190314778A1 - Status: Published application - Kind: A1 - Application number: 16/450,113 - Filed: 2019-06-24 - Published: 2019-10-17 - Earliest claimed: 2016-12-23 - Assignees: T&R Biofab Co., Ltd.; Korea Polytechnic University Industry Academic Cooperation Foundation - Applicants: T&R Biofab Co, Ltd.; Korea Polytechnic University Industry Academic Cooperation Foundation - Inventors: Geunseon Ahn; Jinhyung Shim; Jeongseok Lee; lngyu Lee; Kyunghyun Min; Songwan Jin; Wonsoo Yun - Web page: https://patentgrep.com/patent/US20190314778A1 ## CPC - B01F 35/7174: {using pistons, plungers or syringes} - B01F 27/90: with paddles or arms - B01F 27/92: with helices or screws - B01F 35/32005: {Type of drive} - B01F 35/50: Mixing receptacles - B05C 17/00566: {with a dynamic mixer in the nozzle} - B33Y 40/10: Pre-treatment - C12M 27/02: {Stirrer or mobile mixing elements} - B01F 35/33: Transmissions; Means for modifying the speed or direction of rotation - B01F 2101/44: Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation - B05C 17/0133: {Nut and bolt advancing mechanism, e.g. threaded piston rods} - B33Y 30/00: Apparatus for additive manufacturing; Details thereof or accessories therefor - C12M 33/00: {Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus (chemical or physical laboratory apparatus in general B01L, devices for taking cell samples A61B10/0045, withdrawing or distributing predetermined quantities of fluid B01L99/00)} - B01F: MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING (mixing paints B44D3/06) - B05C: APPARATUS FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL (spraying apparatus, atomising apparatus, nozzles B05B; plant for applying liquids or other fluent materials to objects by electrostatic spraying B05B5/08) - B33Y: ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract The mixing device of cell printing composition according to the present disclosure comprises a first syringe comprising a first container for containing first liquid, a first moving element discharging the first liquid contained in the first container; a second syringe comprising a second container for containing second liquid, a second moving element discharging the second liquid contained in the second container; a mixing container which comprises a rotating mixing unit and receives the discharged first liquid and the discharged second liquids; a power transmission unit for driving the first moving element, the second moving element and the mixing unit; a first threaded guide where the first moving element is movably connected; a second threaded guide where the second moving element is movably connected; and a rod for rotating the mixing unit. ## Claims ### Claim 1 A mixing device of cell printing composition comprising: a first syringe comprising a first container for containing first liquid, a first moving element discharging the first liquid contained in the first container; a second syringe comprising a second container for containing second liquid, a second moving element discharging the second liquid contained in the second container; a mixing container comprising a rotating mixing unit, the mixing container receiving the discharged first liquid and the discharged second liquid; a power transmission unit for driving the first moving element, the second moving element and the mixing unit; a first threaded guide where the first moving element is movably connected; a second threaded guide where the second moving element is movably connected; and a rod for rotating the mixing unit; wherein the first moving element moves by the rotation of the first threaded guide; the second moving element moves by the rotation of the second threaded guide; and the first threaded guide, the second threaded guide, and the rod are rotated by the power transmission unit. ### Claim 2 (depends on 1) The mixing device according to claim 1, wherein the power transmission unit comprises a ring gear, a sun gear which is rotatable by the rotation of the ring gear, a first planetary gear which is engageable with the sun gear, and a second planetary gear which is engageable with the sun gear; and wherein the first threaded guide is rotatable by the first planetary gear; the second threaded guide is rotatable by the second planetary gear; and the rod is rotatable by the sun gear. ### Claim 3 (depends on 2) The mixing device according to claim 2, wherein the first moving element is connected to the first threaded guide via a first connecting portion which is guidable by the first threaded guide; and the second moving element is connected to the second threaded guide via a second connecting portion which is guidable by the second threaded guide. ### Claim 4 (depends on 3) The mixing device according to claim 3, further comprising a first guard portion having a first slot where the first connecting portion is guidable; and a second guard portion having a second slot where the second connecting portion is guidable. ### Claim 5 (depends on 1) The mixing device according to claim 1, wherein the mixing unit comprises a second mixing portion comprising a spiral portion extending up and down direction. ### Claim 6 (depends on 5) The mixing device according to claim 5, wherein the second mixing portion further comprises a shaft extending up and down direction; the spiral portion extends in the longitudinal direction of the shaft; and the shaft is connected to the rod and is rotatable by the rotation of the rod. ### Claim 7 (depends on 1) The mixing device according to claim 1, wherein the first moving element is slidable within the first container; and the second moving element is slidable within the second container. ### Claim 8 (depends on 4) The mixing device according to claim 4, wherein the first connecting portion comprises a first groove where the first guard is fit; and the second connecting portion comprises a second groove where the second guard is fit. ### Claim 9 (depends on 1) The mixing device according to claim 1, wherein any one of the first liquid and the second liquid is cell culture fluid and the other is hydrogel having a sufficient viscosity for cell printing.