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US 2019/0310260 A1Published application
Regeneratable Biosensor and Methods of Use Thereof
AssigneeThe Charles Stark Draper Laboratory, Inc.
InventorsStephanie Angione, Madeline Cooper, Jonathan Coppeta, Thomas Mulhern, Hesham Azizgolshani
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Abstract
A multiplex-able, regeneratable nucleic-acid linked immunoassay method and system for the detection of a single specific, or multiple, soluble analytes in solution and regeneratable biosensor devices for same are described.
Claims10
A method of detecting an analyte, or a plurality of analytes, of interest in a fluid sample, the method comprising:
- a) contacting a fluid sample containing the analyte of interest with a regeneratable biosensor for a time sufficient and under conditions sufficient for the analyte of interest to bind to the capture element of the capture element-oligo conjugate, thereby forming an analyte-capture element-oligo conjugate complex immobilized on the solid surface;
- b) contacting the analyte-capture element-oligo conjugate of step a) with a detectable reagent that specifically reacts with/binds to the analyte of interest for a time sufficient and under conditions for the detectable reagent to react with the analyte;
- and
- c) detecting the reagent, thereby detecting the analyte of the analyte-capture element-oligo conjugate.
1 more element
A method of detecting an analyte, or a plurality of analytes, of interest in a fluid sample, the method comprising:
- a) contacting a fluid sample containing the analyte of interest with a regeneratable biosensor for a time sufficient and under conditions sufficient for the analyte of interest to bind to the antibody of the antibody-oligo conjugate, thereby forming an analyte-antibody-oligo conjugate complex immobilized on the streptavidin surface;
- b) contacting the analyte-antibody-oligo conjugate of step a) with a detectable reagent that specifically reacts with/binds to the analyte of interest for a time sufficient and under conditions for the detectable reagent to react with the analyte;
- and
- c) detecting the reagent, thereby detecting the analyte of the analyte-antibody-oligo conjugate.
1 more element
Dependencies are inferred from the claim text.