Method for producing image of biological sample and optical system using same

A method for producing image of biological sample, comprising steps of:

• inputting a grayscale reflection image or a grayscale interference image of a biological sample into a first memory block of an information processing apparatus, wherein the grayscale reflection image or the grayscale interference image has a first image resolution, and inputting a grayscale fluorescent image of the biological sample into a second memory block of the information processing apparatus, wherein the grayscale fluorescent image has a second image resolution, and the first image resolution is equal to or different from the second image resolution;
• using the information processing apparatus to convert the grayscale reflection image or the grayscale interference image into an RGB reflection image or an RGB interference image by performing a first color transform operation, and using the information processing apparatus to convert the grayscale fluorescent image into an RGB fluorescent image by performing a second color transform operation;
• using the information processing apparatus to perform an image fusion operation and an intensity inversion operation on the RGB reflection image and the RGB fluorescent image or on the RGB interference image and the RGB fluorescent image to generate a pseudo H&E image;
• and
• outputting the pseudo H&E image to a display unit.