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US 2019/0300929 A1Published application
Reaction accelerating agent
AssigneeKikkoman Corporation
InventorsAtsushi ICHIYANAGI, Kazuhiko SHIMOJI
Life of this patent
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Abstract
Provided is a compound capable of accelerating the enzymatic reaction catalyzed by peroxidase. The present invention provides a peroxidase reaction accelerating agent comprising a compound represented by formula (I) and a method for measuring hydrogen peroxide using the same.
Claims16
A method for measuring hydrogen peroxide, comprising the step of using peroxidase and a peroxidase reaction accelerating agent represented by the following formula (I):
- [Formula 1]
- R1—N═N—R2 (I)
- wherein
- R1 and R2 are each independently an aromatic 6-membered monocyclic carbocyclic ring, or a 10-membered fused ring containing the carbocyclic ring, wherein the ring may optionally be substituted with one or more substituents, wherein
- the substituent(s) is selected from the group consisting of —F, —Cl, —Br, —I, —At, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, ═O, —OH, —O—C1-6 alkyl, —CONH2, —NO2, —NH2, —CO—NH2, —R3, —NHCO—NH—R5—N═N—R6, —SO3X, —COOX, Y, and Z;
- X is selected from the group consisting of —H, —Na, —K, and —Li;
- Y is selected from the group consisting of —H, —SO3X, and —COOX;
- Z is selected from the group consisting of —H, —C1-6 alkyl, —O—C1-6 alkyl, and —SO3X;
- —R3 is —H or —NHCO—NH—R5—N═N—R6, or is an aromatic 6-membered monocyclic carbocyclic ring, or a 10-membered fused ring containing the carbocyclic ring, wherein the ring may optionally be substituted with one or more substituents selected from the group consisting of —F, —Cl, —Br, —I, —At, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, ═O, —OH, —O—C1-6 alkyl, —CONH2, —NO2, —NH2, and —SO3X;
- and
- —R5 and —R6 are each independently an aromatic 6-membered monocyclic carbocyclic ring, or a 10-membered fused ring containing the carbocyclic ring, wherein the ring may optionally be substituted with one or more substituents selected from the group consisting of —F, —Cl, —Br, —I, —At, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, ═O, —OH, —O—C1-6 alkyl, —O—CH3, —CONH2, —NO2, —NH2, —SO3X, —COOX, Y, and Z.
8 more elements
A peroxidase reaction accelerating agent comprising a compound represented by formula (I):
- [Formula 14]
- R1—N═N—R2 (I)
- wherein
- R1 and R2 are each independently an aromatic 6-membered monocyclic carbocyclic ring, or a 10-membered fused ring containing the carbocyclic ring, wherein the ring may optionally be substituted with one or more substituents, wherein
- the substituent(s) is selected from the group consisting of —F, —Cl, —Br, —I, —At, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, ═O, —OH, —O—C1-6 alkyl, —CONH2, —NO2, —NH2, —CO—NH2, —R3, —NHCO—NH—R5—N═N—R6, —SO3X, —COOX, Y, and Z;
- X is selected from the group consisting of —H, —Na, —K, and —Li;
- Y is selected from the group consisting of —H, —SO3X, and —COOX;
- Z is selected from the group consisting of —H, —C1-6 alkyl, —O—C1-6 alkyl, and —SO3X;
- —R3 is —H or —NHCO—NH—R5—N—N—R6, or is an aromatic 6-membered monocyclic carbocyclic ring, or a 10-membered fused ring containing the carbocyclic ring, wherein the ring may optionally be substituted with one or more substituents selected from the group consisting of —F, —Cl, —Br, —I, —At, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, ═O, —OH, —O—C1-6 alkyl, —CONH2, —NO2, —NH2, and —SO3X;
- and
- —R5 and —R6 are each independently an aromatic 6-membered monocyclic carbocyclic ring, or a 10-membered fused ring containing the carbocyclic ring, wherein the ring may optionally be substituted with one or more substituents selected from the group consisting of —F, —Cl, —Br, —I, —At, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, ═O, —OH, —O—C1-6 alkyl, —O—CH3, —CONH2, —NO2, —NH2, —SO3X, —COOX, Y, and Z.
8 more elements
Dependencies are inferred from the claim text.