Microorganisms and methods for the production of biosynthesized target products having reduced levels of byproducts
AssigneeGenomatica, Inc.
InventorsRobin E. OSTERHOUT, Priti PHARKYA
Life of this patent
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Abstract
Provided herein are non-naturally occurring microbial organisms having biosynthetic pathways for production of target products and one or more genetic modifications that reduce a byproduct of the biosynthetic pathway. Compositions of target products from such cells and methods of using such cells are provided.
Claims27
A genetically modified cell capable of producing a target product, said target product comprising hexamethylenediamine (HMD), levulinic acid (LVA), 6-aminocaproic acid (6ACA), caprolactam (CPL), caprolactone (CPO), adipic acid (ADA), or 1,6-hexanediol (HDO) or a combination thereof, wherein said genetically modified cell comprises one or more genetic modifications selected from:
- (a) a genetic modification that decreases activity of an enzyme selected from an Oxidoreductase acting on an aldehyde or oxo moiety (A1);
- Oxidoreductase acting on a acyl-CoA moiety (A2);
- Oxidoreductase acting on an aldehyde moiety (A3);
- Oxidoreductase acting on an aldehyde or acyl-CoA moiety (A4);
- Aldehyde oxidase acting on an aldehyde moiety (A5);
- Oxidoreductase acting on an alkene or alkane moiety (A6);
- Oxidoreductase acting on an amine moiety (A7);
- Amine N-methyltransferase acting on an amine moiety (A8);
- Carbamoyl transferase acting on an amine moiety (A9);
- Acyltransferase acting on an acyl-CoA moiety (A10);
- Acyltransferase acting on an amine or acyl-CoA moiety (A11);
- N-propylamine synthase acting on an amine moiety (A12);
- Aminotransferase acting on an amine or aldehyde moiety (A13);
- CoA transferase acting on an acyl-CoA or an acid moiety (A14);
- Thioester hydrolase acting on an acyl-CoA moiety (A15);
- Decarboxylase acting on an oxoacid moiety (A16);
- Dehydratase acting on a hydroxyacid moiety (A17);
- Ammonia-lyase acting on an amine moiety (A18);
- CoA ligase acting on an acyl-CoA or acid moiety (A19);
- glutamyl:amine ligase acting on an amine moiety (A20);
- Amine hydroxylase acting on an amine moiety (A21);
- Oxidoreductase acting on an acyl-CoA moiety (A22);
- Amine oxidase acting on an amine moiety (A23);
- short chain diamine exporter acting on a diamine moiety (A24);
- and putrescine permease acting on a diamine moiety (A25);
- (b) a genetic modification that increases activity of an enzyme selected from Amide hydrolase or amidase acting on an amide moiety (B1);
- Cyclic amide hydrolase or lactamase acting on a cyclic amide moiety (B2);
- CoA ligase acting on an acid moiety (B3);
- Diamine transporter (longer chain diamines) acting on an amine moiety (B4);
- and diamine permease acting on an amine moiety (B5);
- and
- (c) a combination of two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or all of the genetic modifications of (a) and (b);
- wherein said cell produces a reduced amount of one or more byproducts when compared to a cell without said one or more genetic modifications.
-24. (canceled)
-53. (canceled)
(canceled)
-107. (canceled)
A composition comprising a target product selected from LVA, 6ACA, CPL, CPO, ADA, HMD or HDO and a byproduct selected from Table 10 or Table 11.
-112. (canceled)
-118. (canceled)
-160. (canceled)
Dependencies are inferred from the claim text.