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US 2019/0295686 A1Published application
System for determination of temperature cycling protocols for polymerase chain reactions: dna melting
AssigneeChakrabarti Advanced Technology LLC
InventorRaj CHAKRABARTI
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Abstract
In one aspect, methods are described herein for enhancing one or more nucleic acid interactions. For example, in some embodiments, methods of enhancing one or more steps of polymerase chain reaction (PCR) are described herein. In some embodiments, the optimal temperature cycling protocol for one or more PCR cycles can be determined according to methods described herein.
Claims5
A system for determining melting temperature and time in a temperature cycling protocol for a PCR reaction to achieve amplification of a DNA concentration in excess of a predetermined threshold, the system comprising:
- a memory device;
- and a processor coupled to the memory device, the processor configured to:
- a) receive information comprising concentrations of double stranded DNA (dsDNA) and a predetermined melting temperature or time in the PCR reaction;
- b) determine a forward rate constant, km(T), for melting the complete sequence of the dsDNA as a function of temperature T;
- c) determine a reverse rate constant, k−m(T), for melting the complete sequence of the dsDNA as a function of temperature T;
- and
- d) determine
- (I) a temperature for DNA melting sufficient to produce the concentration of DNA in excess of the predetermined threshold within a predetermined extension time period or
- (II) a time period for DNA melting sufficient to produce the concentration of DNA in excess of the predetermined threshold at a predetermined extension temperature.
6 more elements
Dependencies are inferred from the claim text.