Lipid probes and uses thereof
AssigneeThe Scripps Research Institute
InventorsBenjamin F. Cravatt, Micah J. Niphakis, Kenneth Lum, Bruno Correia, Armand Cognetta, Jonathan Hulce
Life of this patent
Estimating the term…
Abstract
Disclosed herein are methods, compositions, probes, assays and kits for identifying a lipid binding protein as a drug binding target. Also disclosed herein are methods, compositions, and probes for mapping a ligand binding site on a lipid binding protein, identification of lipid binding proteins, generating drug-lipid binding protein profiles, high throughput drug screening, and identification of drugs as potential lipid binding protein ligands.
Claims21
-29. (canceled)
A process of generating a lipid probe-protein composition, comprising:
- contacting a sample with a lipid probe;
- and
- treating the sample comprising the lipid probe by a photoreactive means to generate the lipid probe-protein composition, wherein the treating time is from about 5 minutes to about 1 hour;
- and
- wherein the lipid probe is a lipid probe of Formula (I):
- wherein:
- A is C16-C20alkyl or C16-C20alkenyl;
- D is —OH, —NH2, —NHR7, or —OR;
- R7 is C1-C4alkyl, (C1-C5alkyl)OH, or (C1-C5)SO3M;
- R8 is (CH2OH)n;
- M is monovalent or divalent cation;
- n is 1, 2, or 3;
- PG is a photoreactive group;
- and
- AH is an affinity handle;
- and
- wherein PG is attached to A or D and AH is attached to A or D.
A process of generating a lipid probe-protein composition, comprising:
- contacting a sample with a lipid probe, wherein the lipid probe is selected from Table 1;
- and
- treating the sample comprising the lipid probe by a photoreactive means to generate the lipid probe-protein composition, wherein the treating time is from about 5 minutes to about 1 hour.
Dependencies are inferred from the claim text.