Cell capture system and method of use
AssigneeCelsee Diagnostics, Inc.
InventorKalyan Handique
Life of this patent
Estimating the term…
Abstract
A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
Claims20
A method for automatically processing target particles in single-particle format, the method comprising:
- at a workstation, receiving a substrate comprising a network having an input channel at an upstream end, an output channel at a downstream end, and a set of structures in communication with the input channel and the output channel;
- at the workstation, retaining a container comprising a set of target particles;
- from a controller in communication with a handling subsystem of the workstation, receiving a set of instructions for automatic processing of the set of target particles, in single-particle format;
- and
- based upon the set of instructions:
- with the handling subsystem, transmitting the set of target particles into the network;
- isolating each of the set of target particles in single-particle format with an isolation material, within the network of the substrate, and
- delivering, by way of the handling subsystem, a reagent material from a reagent container into the input channel of the substrate.
A method for automatically processing target particles in single-particle format, the method comprising:
- at a workstation, receiving a substrate comprising a network having an input channel at an upstream end, an output channel at a downstream end, and a set of structures in communication with the input channel and the output channel;
- at the workstation, retaining a sample comprising a set of target cells;
- at the workstation, retaining a volume comprising a set of functionalized particles;
- from a controller in communication with a handling subsystem of the workstation, receiving a set of instructions for automatic processing of the set of target cells, in single cell format;
- and
- based upon the set of instructions:
- with the handling subsystem, transmitting the sample and the volume into the network;
- co-capturing the set of target cells with the set of functionalized particles within the network in single-particle format;
- isolating the set of target cells with an isolation material, within the network, and
- deivering, by way of the handling subsystem, a reagent material from a reagent container into the input channel of the substrate.
Dependencies are inferred from the claim text.